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The intramammary efficacy of first generation cephalosporins against Staphylococcus aureus mastitis in mice.

Abstract
Staphylococcus aureus-induced mastitis in cattle causes important financial losses in the dairy industry due to lower yield and bad milk quality. Although S. aureus is susceptible to many antimicrobials in vitro, treatment often fails to cure the infected udder. Hence, comprehensive evaluation of antimicrobials against S. aureus mastitis is desirable to direct treatment strategies. The mouse mastitis model is an elegant tool to evaluate antimicrobials in vivo while circumventing the high costs associated with bovine experiments. An evaluation of the antimicrobial efficacy of the intramammary (imam) applied first generation cephalosporins cefalexin, cefalonium, cefapirin and cefazolin, was performed using the S. aureus mouse mastitis model. In vivo determination of the effective dose 2log(10) (ED(2log10)), ED(4log10), protective dose 50 (PD(50)) and PD(100) in mouse mastitis studies, support that in vitro MIC data of the cephalosporins did not fully concur with the in vivo clinical outcome. Cefazolin was shown to be the most efficacious first generation cephalosporin to treat S. aureus mastitis whereas the MIC data indicate that cefalonium and cefapirin were more active in vitro. Changing the excipient for imam application from mineral oil to miglyol 812 further improved the antimicrobial efficacy of cefazolin, confirming that the excipient can influence the in vivo efficacy. Additionally, statistical analysis of the variation of S. aureus-infected, excipient-treated mice from fourteen studies emphasizes the strength of the mouse mastitis model as a fast, cost-effective and highly reproducible screening tool to assess the efficacy of antimicrobial compounds against intramammary S. aureus infection.
AuthorsDieter Demon, Carolin Ludwig, Koen Breyne, David Guédé, Julia-Charlotte Dörner, Robrecht Froyman, Evelyne Meyer
JournalVeterinary microbiology (Vet Microbiol) Vol. 160 Issue 1-2 Pg. 141-50 (Nov 09 2012) ISSN: 1873-2542 [Electronic] Netherlands
PMID22677480 (Publication Type: Journal Article)
CopyrightCopyright © 2012 Elsevier B.V. All rights reserved.
Chemical References
  • Anti-Infective Agents
  • Cephalosporins
Topics
  • Animals
  • Anti-Infective Agents (pharmacology)
  • Cattle
  • Cephalosporins (pharmacology)
  • Disease Models, Animal
  • Female
  • Lactation
  • Mastitis, Bovine (drug therapy)
  • Mice
  • Microbial Sensitivity Tests (veterinary)
  • Staphylococcal Infections (drug therapy, microbiology, prevention & control, veterinary)
  • Staphylococcus aureus (drug effects)

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