[Anti-tumor peptide of tumstatin in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice].

Abstract

OBJECTIVE:

To study the effect of anti-tumor peptide of tumstatin on tumor growth of human laryngeal squamous carcinoma in nude mice and the underlying mechanism.

METHOD:

Nude mice model bearing laryngocarcinoma were established by using human laryngeal squamous carcinoma cell line (Hep-II). The animals were given tumstatin or PBS for 10 consecutive days. The volumes of the subcutaneous tumor were observed. The microstructure in which the general 2-step immunohistochemical examination was adopted and ultra-micro-structural changes of carcinoma after administration of tumstatin were observed under light and electron microscopes for pathology examination.

RESULT:

The differences was statistically significant in the net mice weight, tumor weight, tumor volume and tumor weight/net mice weight between the treatment group and the control group (P<0.01). The restrained percentage of tumor was 51.58%. The necrosis and apoptosis of the tumor cells and the angiogenesis reduction were found under light and electron microscope in the treatment group. MVD of the treatment group was lower than that of the control group (P<0.01).

CONCLUSION:

Tumstatin can significantly restrain the development of laryngocarcinoma.

• Authors: Chao Wang, Qiuying Li, Hongchao Yao, Ming Liu, Yuli Xiao, Dejun Jin
• Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery (Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 26 Issue 6 Pg. 277-9 (Mar 2012) ISSN: 2096-7993 [Print] China
• PMID: 22675939 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Autoantigens
• Collagen Type IV
• Peptides
• type IV collagen alpha3 chain

Topics

• Animals
• Apoptosis (drug effects)
• Autoantigens (pharmacology)
• Carcinoma, Squamous Cell (pathology)
• Cell Line, Tumor
• Collagen Type IV (pharmacology)
• Female
• Humans
• Laryngeal Neoplasms (pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Peptides (pharmacology)
• Xenograft Model Antitumor Assays