Fargesin improves lipid and glucose metabolism in 3T3-L1 adipocytes and high-fat diet-induced obese mice.

Abstract

This study examined the effects of fargesin, a neolignan isolated from Magnolia plants, on obesity and insulin resistance and the possible mechanisms involved in these effects in 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. Fargesin promoted the glucose uptake in 3T3-L1 adipocytes. In HFD-induced obese mice, fargesin decreased the body weight gain, white adipose tissue (WAT), and plasma triglyceride, non-esterified fatty acid and glucose levels, and improved the glucose tolerance. Fargesin increased glucose transporter 4 (GLUT4) protein expression and phosphorylation of Akt, AMP-activated protein kinase (AMPK), and acetyl-CoA carboxylase (ACC) in both 3T3-L1 adipocytes and WAT of HFD-induced obese mice. Fargesin also decreased the mRNA expression levels of fatty acid oxidation-related genes, such as peroxisome proliferator-activated receptor α (PPARα), carnitine palmitoyltransferase-1 (CPT-1), uncoupling protein-2 (UCP-2) and leptin in WAT. Taken together, the present findings suggest that fargesin improves dyslipidemia and hyperglycemia by activating Akt and AMPK in WAT.

• Authors: Young-Sil Lee, Byung-Yoon Cha, Sun-Sil Choi, Yumiko Harada, Bong-Keun Choi, Takayuki Yonezawa, Toshiaki Teruya, Kazuo Nagai, Je-Tae Woo
• Journal: BioFactors (Oxford, England) (Biofactors) 2012 Jul-Aug Vol. 38 Issue 4 Pg. 300-8 ISSN: 1872-8081 [Electronic] Netherlands
• PMID: 22674784 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 International Union of Biochemistry and Molecular Biology, Inc.

Chemical References

• Benzodioxoles
• Blood Glucose
• Glucose Transporter Type 4
• Hypoglycemic Agents
• Hypolipidemic Agents
• Lignans
• Lipids
• Slc2a4 protein, mouse
• Proto-Oncogene Proteins c-akt
• Adenylate Kinase
• Acetyl-CoA Carboxylase
• fargesin
• Glucose

Topics

• 3T3-L1 Cells
• Acetyl-CoA Carboxylase (metabolism)
• Adenylate Kinase (metabolism)
• Adipocytes (drug effects, metabolism)
• Animals
• Benzodioxoles (pharmacology, therapeutic use)
• Blood Glucose
• Diet, High-Fat (adverse effects)
• Gene Expression (drug effects)
• Glucose (metabolism)
• Glucose Transporter Type 4 (metabolism)
• Hypoglycemic Agents (pharmacology, therapeutic use)
• Hypolipidemic Agents (pharmacology, therapeutic use)
• Lignans (pharmacology, therapeutic use)
• Lipid Metabolism (drug effects)
• Lipids (blood)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Obese
• Obesity (drug therapy, etiology, metabolism)
• Organ Size (drug effects)
• Phosphorylation
• Protein Processing, Post-Translational
• Proto-Oncogene Proteins c-akt (metabolism)
• Weight Gain (drug effects)