Lectin histochemistry of the central nervous system in a case of feline alpha-mannosidosis.

Abstract

The diagnosis of feline alpha-mannosidosis is made by demonstrating deficient activity of the enzyme alpha-mannosidase or an elevation of its undergraded substrate in body fluids or tissue. In this study the storage of specific sugar residues in the brain and spinal cord in a case of feline alpha-mannosidosis was examined by means of nine different biotinylated lectins and the avidin-biotin-peroxidase method on formalin-fixed, paraffin-embedded tissue sections. The lectin staining pattern strongly correlated with the known biochemical findings of the stored oligosaccharides in feline alpha-mannosidosis and was different from the lectin reactivity of normal cat tissues. This confirms that lectin histochemistry is a simple, relatively inexpensive and reliable method for diagnosing alpha-mannosidosis in cats.

• Authors: M Castagnaro
• Journal: Research in veterinary science (Res Vet Sci) Vol. 49 Issue 3 Pg. 375-7 (Nov 1990) ISSN: 0034-5288 [Print] England
• PMID: 2267428 (Publication Type: Journal Article)

Chemical References

• Lectins
• Mannosidases
• alpha-Mannosidase

Topics

• Animals
• Cat Diseases (diagnosis, pathology)
• Cats
• Central Nervous System (chemistry)
• Cerebellum (chemistry)
• Cerebral Cortex (chemistry)
• Histocytochemistry
• Lectins
• Mannosidases (deficiency)
• Medulla Oblongata (chemistry)
• Neurons (chemistry)
• Spinal Cord (chemistry)
• alpha-Mannosidase
• alpha-Mannosidosis (diagnosis, pathology, veterinary)