Abstract |
Antigenic diversity among different hantaviruses requires a variety of reagents for diagnosis of hantavirus infection. To develop a diagnostic method applicable to various hantavirus infections with a single set of reagents, we developed an enzyme-linked immunosorbent assay (ELISA) using recombinant nucleocapsid proteins of three hantaviruses, Amur, Hokkaido, and Sin Nombre viruses. This novel cocktail antigen-based ELISA enabled detection of antibodies against Hantaan, Seoul, Amur, Puumala, and Sin Nombre viruses in immunized laboratory animals. In wild rodent species, including Apodemus, Rattus, and Myodes, our ELISA detected antibodies against hantaviruses with high sensitivity and specificity. These data suggest that our novel diagnostic ELISA is a useful tool for screening hantavirus infections and could be effectively utilized for serological surveillance and quarantine purposes.
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Authors | Takahiro Sanada, Hiroaki Kariwa, Ngonda Saasa, Keisuke Yoshikawa, Takahiro Seto, Vyacheslav G Morozov, Evgeniy A Tkachenko, Leonid I Ivanov, Kumiko Yoshimatsu, Jiro Arikawa, Kentaro Yoshii, Ikuo Takashima |
Journal | The Journal of veterinary medical science
(J Vet Med Sci)
Vol. 74
Issue 9
Pg. 1237-42
(Sep 2012)
ISSN: 1347-7439 [Electronic] Japan |
PMID | 22673703
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Nucleocapsid Proteins
- Recombinant Proteins
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Topics |
- Animals
- Antibodies, Viral
(isolation & purification)
- Arvicolinae
- Enzyme-Linked Immunosorbent Assay
(methods, veterinary)
- Orthohantavirus
(genetics)
- Hantavirus Infections
(diagnosis, immunology, veterinary)
- Murinae
- Nucleocapsid Proteins
(genetics)
- Rats
- Recombinant Proteins
(genetics)
- Rodent Diseases
(diagnosis, immunology, virology)
- Sensitivity and Specificity
- Serologic Tests
(veterinary)
- Species Specificity
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