Abstract | OBJECTIVES: METHODS: The Wistar rats (280-320 g) were divided into control, 12 and 36 hours after TBI groups (n = 3) and their TBI model was established. Subsequently, NGB expression level was examined by Western blot and immunohistochemical. Beyond that, adenoviral vectors pAdEasy-rNGB-GFP and pAdEasy-GFP were constructed and transfected into the rat brain respectively (pAdEasy-GFP was control), and the neuroprotective effects were examined by immunohistochemical. RESULTS: Immunohistochemical and Western blot results demonstrated that NGB expression level was increased at 12 and 36 hours after TBI injury compare with control. Meanwhile, the pAdEasy-rNGB-GFP transfected rats suffered less necrosis and apoptosis compare to control. CONCLUSIONS: NGB was upregulated in TBI and overexpressed rNGB had a significant neuroprotection in TBI. However, the mechanism remained unknown. This study suggested that rNGB overexpression may be a new strategy for treating of TBI.
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Authors | Aijia Shang, Xingjun Feng, Hangyan Wang, Jing Wang, Xingyi Hang, Ying Yang, Zhongfeng Wang, Dingbiao Zhou |
Journal | Neurological research
(Neurol Res)
Vol. 34
Issue 6
Pg. 588-94
(Jul 2012)
ISSN: 1743-1328 [Electronic] England |
PMID | 22664218
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nerve Tissue Proteins
- Neuroglobin
- Neuroprotective Agents
- Globins
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Topics |
- Animals
- Blotting, Western
- Brain Injuries
(metabolism)
- Disease Models, Animal
- Globins
(biosynthesis, pharmacology)
- Immunohistochemistry
- Nerve Tissue Proteins
(biosynthesis, pharmacology)
- Neuroglobin
- Neuroprotective Agents
(metabolism, pharmacology)
- Rats
- Rats, Wistar
- Transfection
- Up-Regulation
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