[Effect of total glucosides of peony on expression and DNA methylation status of ITGAL gene in CD4(+) T cells of systemic lupus erythematosus].

Abstract

OBJECTIVE:

To investigate the effect of total glucosides of peony (TGP) on expression and DNA methylation status of ITGAL gene (CD11a) in CD4(+) T cells from patients with systemic lupus erythematosus (SLE).

METHODS:

CD4(+) T cells were isolated by positive selection using CD4 beads. CD4(+) T cells were treated by TGP at 0, 62.5, 312.5 and 1562.5 mg/L for 48 h. The MTT method was used to assess cell viability; mRNA expression level was measured by realtime-PCR; protein level of CD11a was measured by flow cytometric analysis; DNA methylation status was assayed by bisulfite sequencing.

RESULTS:

No significant change in cell viability was found in CD4(+) T cells among the different concentration groups (P>0.05). Compared with control, the mRNA and protein levels of ITGAL were down-regulated significantly in SLE CD4(+) T cells treated with TGP (1562.5 mg/L) (P< 0.01). Furthermore, the extent of DNA methylation of ITGAL promoter was increased in TGP (1562.5 mg/L) treated CD4(+) T cells compared with control group (P<0.01).

CONCLUSION:

TGP can repress CD11a gene expression through enhancing DNA methylation of ITGAL promoter in CD4(+) T cells from patients with SLE. This observation represents a preliminary step in understanding the mechanism of TGP in SLE therapy.

• Authors: Ming Zhao, Gongping Liang, Shuangyan Luo, Qianjin Lu
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 37 Issue 5 Pg. 463-8 (May 2012) ISSN: 1672-7347 [Print] China
• PMID: 22659657 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD11a Antigen
• Glucosides
• RNA, Messenger

Topics

• CD11a Antigen (genetics, metabolism)
• CD4-Positive T-Lymphocytes (immunology, metabolism)
• DNA Methylation (drug effects)
• Down-Regulation (drug effects)
• Glucosides (pharmacology)
• Humans
• Lupus Erythematosus, Systemic (genetics, immunology)
• Paeonia (chemistry)
• Promoter Regions, Genetic (genetics)
• RNA, Messenger (genetics, metabolism)