Xiao-Qing-Long-Tang attenuates allergic airway inflammation and remodeling in repetitive Dermatogoides pteronyssinus challenged chronic asthmatic mice model.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Xiao-Qing-Long-Tang (XQLT) has been used for centuries in Asia to effectively treat patients with bronchial asthma.

AIM OF THE STUDY:

We previously found that single and multiple doses of XQLT administered to sensitized mice before allergen challenge resulted in suppressed airway hyper-responsiveness and airway inflammation. In this study we aimed to investigate whether XQLT has the potential to attenuate the severity of asthma symptoms, and immunomodulatory mechanism of XQLT in a repetitive Dermatogoides pteronyssinus (D. pteronyssinus)-challenged chronic asthmatic mice model.

MATERIALS AND METHODS:

BALB/c mice were intratracheally (i.t.) inoculated with five doses of D. pteronyssinus (50 μl, 1mg/ml) and orally administered of XQLT (1 g/kg) at 1-week intervals. At three days after the last challenge, mice were sacrificed to evaluate airway remodeling, inflammation, lung histological features, and the expression profiles of cytokines and various genes.

RESULTS:

XQLT significantly reduced bronchial inflammatory cell infiltration and airway remodeling. It inhibited D. pteronyssinus-induced total IgE and D. pteronyssinus-specific IgG1 in serum, and changed the "T(H)2-bios" in BALF by inhibiting the activation of NF-κB. Collagen assay and Histopathology indicated that XQLT reduced airway remodeling in the lung. Simultaneously, the RT-PCR analysis showed that XQLT downregulated IL-10, IL-13, RANTES, Eotaxin, and MCP-1 mRNA expression in the lung. Moreover, EMSA and immunohistochemistry staining demonstrated that XQLT inhibited NF-κB expression in the nucleus of bronchial epithelial cells.

CONCLUSIONS:

These results suggest that XQLT exhibits anti-airway inflammatory, anti-airway remodeling, and specific immunoregulatory effects in a chronic asthmatic mice model.

• Authors: Shulhn-Der Wang, Li-Jen Lin, Chih-Lung Chen, Shih-Chang Lee, Chin-Che Lin, Jiu-Yao Wang, Shung-Te Kao
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 142 Issue 2 Pg. 531-8 (Jul 13 2012) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 22658987 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Anti-Asthmatic Agents
• Chemokine CCL5
• Chemokines, CC
• Drugs, Chinese Herbal
• Immunoglobulin G
• Interleukin-13
• NF-kappa B
• sho-seiryu-to
• Interleukin-10
• Immunoglobulin E

Topics

• Airway Remodeling (drug effects)
• Animals
• Anti-Asthmatic Agents (pharmacology, therapeutic use)
• Asthma (drug therapy, immunology, metabolism, pathology)
• Bronchial Hyperreactivity (drug therapy, immunology, metabolism)
• Cell Nucleus (metabolism)
• Chemokine CCL5 (metabolism)
• Chemokines, CC (metabolism)
• Chronic Disease
• Disease Models, Animal
• Down-Regulation
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Epithelial Cells (metabolism)
• Immunoglobulin E (blood)
• Immunoglobulin G (blood)
• Inflammation (drug therapy, metabolism)
• Interleukin-10 (metabolism)
• Interleukin-13 (metabolism)
• Lung (drug effects, immunology, metabolism, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• NF-kappa B (metabolism)
• Phytotherapy
• Pyroglyphidae