Abstract | INTRODUCTION: METHODS: Twenty-two young male farm pigs were anesthetized and subjected to 45 mins of ischemia and 3 and a half hours of reperfusion, in the left circumflex coronary artery territory, via the inflation and deflation of an angioplasty balloon. Animals were randomly assigned to receive either an intravenous infusion of 100 mg/ kg ascorbic acid and 60 mg/kg desferrioxamine (treatment group, TG) or an equal amount of normal saline (control group, CG). The I/R ratio, the ratio of the infarcted (necrotic) zone (I) to the myocardial area at risk (R) after 3 and a half hours of reperfusion, was calculated using the tetrazolium staining method. Left ventricular end diastolic pressure (LVEDP), number of episodes of ventricular arrhythmias, TIMI flow in the reperfused vessel, and left ventricular ejection fraction (LVEF) were evaluated within the first hour post reperfusion in order to assess further injury severity. RESULTS: There was no significant difference in the I/R between the TG (27.9 ± 2.2%) and the CG (32.9 ± 2.4%) (p=0.15). In both groups there was a significant reduction in LVEF (-11.6 ± 2.28% for TG and -12.0 ± 2.27% for CG, p<0.01 for both groups) and a significant increase in LVEDP (+3.2 ± 0.9 mmHg for TG and +4.6 ± 0.9 mmHg for CG, p<0.01 for both groups) compared to the baseline values. No significant difference was noted between groups (p=0.61 for LVEF and p=0.60 for LVEDP values, at one hour post reperfusion). In all other parameters measured, no significant difference was observed between the study groups. CONCLUSIONS:
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Authors | Georgios N Chatziathanasiou, Dimitrios N Nikas, Christos S Katsouras, Nikolaos D Kazakos, Vasiliki Bouba, Theodoros Vougiouklakis, Katerina K Naka, Lampros K Michalis |
Journal | Hellenic journal of cardiology : HJC = Hellenike kardiologike epitheorese
(Hellenic J Cardiol)
2012 May-Jun
Vol. 53
Issue 3
Pg. 195-204
ISSN: 2241-5955 [Electronic] Netherlands |
PMID | 22653244
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Deferoxamine
- Ascorbic Acid
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Topics |
- Animals
- Antioxidants
(therapeutic use)
- Arrhythmias, Cardiac
- Ascorbic Acid
(therapeutic use)
- Coronary Vessels
(physiopathology)
- Deferoxamine
(therapeutic use)
- Disease Models, Animal
- Drug Therapy, Combination
- Infusions, Intravenous
- Male
- Myocardial Infarction
(drug therapy, pathology, physiopathology)
- Myocardial Reperfusion Injury
(physiopathology, prevention & control)
- Myocardium
(pathology)
- Random Allocation
- Stroke Volume
- Sus scrofa
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