β-Glucans are involved in immune-modulation of THP-1 macrophages.

Abstract

SCOPE:

We aimed to examine different immunological aspects of β-glucans derived from different food sources (oat, barley and shiitake) on phorbol myristate acetate (PMA)-differentiated THP-1 macrophages. Commercially purified barley β-glucan (commercial BG) and lentinan were included to compare β-glucans from the same origin but different degree of purity and processing.

METHODS AND RESULTS:

Chemical composition and molecular weight distribution of β-glucan samples were determined. Inflammation-related gene expression kinetics (IL-1β, IL-8, nuclear factor kappa B [NF-κB] and IL-10) after 3, 6 and 24 h of stimulation with 100 μg/mL β-glucan were investigated. All tested β-glucans mildly upregulated the observed inflammation-related genes with differential gene expression patterns. Similar gene expression kinetics, but different fold induction values, was found for the crude β-glucan extracts and their corresponding commercial forms. Pre-incubation of THP-1 macrophages with β-glucans prior to lipopolysaccharide (LPS) exposure decreased the induction of inflammation-related genes compared to LPS treatment. No production of nitric oxide (NO) and hydrogen peroxide (H₂O₂) was detected in β-glucan stimulated THP-1 macrophages. Phagocytic activity was not different after stimulation by β-glucan samples.

CONCLUSION:

Based on these in vitro analyses, it can be concluded that the analysed β-glucans have varying levels of immunomodulating properties, which are likely related to structure, molecular weight and compositional characteristic of β-glucan.

• Authors: Wasaporn Chanput, Marit Reitsma, Lennart Kleinjans, Jurriaan J Mes, Huub F J Savelkoul, Harry J Wichers
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 56 Issue 5 Pg. 822-33 (May 2012) ISSN: 1613-4133 [Electronic] Germany
• PMID: 22648628 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Immunologic Factors
• Lipopolysaccharides
• Plant Extracts
• beta-Glucans
• Nitric Oxide
• Hydrogen Peroxide
• Glycoside Hydrolases
• Tetradecanoylphorbol Acetate

Topics

• Avena (chemistry)
• Cell Differentiation (drug effects)
• Cell Line, Tumor
• Chromatography, Gel
• Gene Expression Regulation (drug effects)
• Glycoside Hydrolases (metabolism)
• Hordeum (chemistry)
• Humans
• Hydrogen Peroxide (metabolism)
• Immunologic Factors (pharmacology)
• Inflammation (drug therapy, genetics)
• Lipopolysaccharides (pharmacology)
• Macrophages (drug effects, immunology)
• Nitric Oxide (metabolism)
• Phagocytosis (drug effects)
• Plant Extracts (analysis, chemistry)
• Shiitake Mushrooms (chemistry)
• Tetradecanoylphorbol Acetate (pharmacology)
• beta-Glucans (pharmacology)