Apoptotic cell death induced by 5-aminolaevulinic acid-mediated photodynamic therapy of hypertrophic scar-derived fibroblasts.

Abstract

OBJECTIVE:

To investigate the effects of 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) on dermal fibroblasts from hypertrophic scars (HSs).

METHODS:

HS samples were obtained from five patients who underwent surgery, and normal skin from healthy donors was used as a control. Dermal fibroblasts were isolated and cultured with various concentrations of ALA for 6 h. Intracellular protoporphyrin IX (PpIX) was measured by confocal microscopy. After 5 h of ALA treatment, cells were irradiated by a red laser (635 nm wavelength) at a power density of 10 mW/cm(2) with an energy density from 0.5 to 4 J/cm(2). Cell survival was measured by a CCK-8 Kit after 24 h. Cell death was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and flow cytometric analysis of annexin V.

RESULTS:

Intracellular PpIX accumulation was observed in fibroblasts from HS patients and healthy donors after ALA treatment. After laser irradiation, viable cells were decreased among both cell types in a dose-dependent manner with energy density. In addition, apoptotic cell death was observed with low dose PDT, whereas necrotic cells were increased by high dose PDT.

CONCLUSION:

HS-derived fibroblasts efficiently accumulate PpIX after ALA treatment and can be eliminated via apoptosis by controlled laser irradiation.

• Authors: Xin Li, Zhao Pin Zhou, Li Hu, Wen Jie Zhang, Wei Li
• Journal: The Journal of dermatological treatment (J Dermatolog Treat) Vol. 25 Issue 5 Pg. 428-33 (Oct 2014) ISSN: 1471-1753 [Electronic] England
• PMID: 22630039 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Photosensitizing Agents
• Aminolevulinic Acid

Topics

• Adolescent
• Adult
• Aminolevulinic Acid (pharmacology)
• Apoptosis (drug effects)
• Cells, Cultured
• Child
• Cicatrix, Hypertrophic (pathology)
• Fibroblasts (drug effects)
• Humans
• Middle Aged
• Photochemotherapy
• Photosensitizing Agents (pharmacology)
• Skin Diseases (pathology)
• Young Adult