[Clinical significance of determination of serum RNase activities in patients with eosinophilia--II. Measurements using polyuridylic acid and polycytidylic acid as substrates].

Abstract

The activities of RNase (RNase-U and RNase-C) were determined in the serum and leukocytes of 277 patients with 14 cases of various kinds of eosinophilia (not less than 10(3)/microliters), 28 cases of chronic myelocytic leukemia (CML), using polyuridylic acid and polycytidylic acid as synthetic substrates according to the method of Raddi et al. Serum RNase-U activity, serum RNase-C activity and the activity ratio (U/C x 10(-3)) were 55 +/- 14 U, 1,280 +/- 235 U and 44 +/- 11 (mean +/- SD), 196 +/- 137, 1,992 +/- 1,134 U and 97 +/- 38, and 110 +/- 50 U, 1,854 +/- 625 U and 65 +/- 13 for normal subjects, eosinophilia and CML (untreated), respectively. U/C ratio in eosinophilia and CML (untreated) showed a highly significant positive correlation (p less than 0.001) with peripheral eosinophil count; the activity of serum RNase-U per cells in the supernatant of eosinophil homogenate rose significantly (p less than 0.001) compared with that of lymphocytes or granulocytes. Besides, serum and eosinophil RNase-U had a similar optimal pH. These results suggested that serum RNase-U in eosinophilia originated mostly from eosinophils and its rise was correlated strongly with the increase in eosinophils.

• Authors: K Maruoka, M Yamanaka, M Misago, K Nakata, J Tsukada, K Nagata, T Sato, N Mori, S Oda, S Chiba
• Journal: Rinsho byori. The Japanese journal of clinical pathology (Rinsho Byori) Vol. 38 Issue 10 Pg. 1161-6 (Oct 1990) ISSN: 0047-1860 [Print] Japan
• PMID: 2262974 (Publication Type: English Abstract, Journal Article)

Chemical References

• Poly U
• Poly C
• Ribonucleases

Topics

• Clinical Enzyme Tests
• Eosinophilia (diagnosis, etiology)
• Hematologic Diseases (complications)
• Humans
• Hydrogen-Ion Concentration
• Kidney Diseases (complications)
• Poly C
• Poly U
• Ribonucleases (blood)