Transforming growth factor beta1, matrix-metalloproteinase-2 and its tissue inhibitor in patients with pseudoexfoliation glaucoma/syndrome.

Abstract

BACKGROUND/AIM:

Transforming growth factor-beta1 (TGF-beta1), oxidative stress and imbalance between matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) may play an important role in pathogenesis of pseudoexfoliation syndrome/glaucoma (PEX Sy/Gl). The aim of the study was to measure concentrations of TGF-beta1, MMP-2, TIMP-2 in the aqueous humor in the examined group, as well as to compare the biochemical findings with the following clinical parameters: degree of chamber angle pigmantation, presence of pseudoexfoliation and the value of intraocular pressure (IOP).

METHODS:

Aqueous samples from 30 patients with cataract, 30 patients with PEX Sy, 36 patients with PEX Gl, and 42 patients with primary open-angle glaucoma (POAG) were collected during phacoemulsification cataract surgery. TGF beta1, MMP-2, TIMP-2 Fluotokine Multi Analyze Profiling kits and Luminex technology were used to simultaneously measure TGF beta1, MMP-2 and TIMP-2.

RESULTS:

TGF-beta1, MMP-2, TIMP-2 were detected in human aqueous from all the groups with the highest level in the group with PEX Gl. Statistically, a significant correlation between the levels of TGF beta1, MMP-2, TIMP-2 in the aqueous humor of the patients with PEX Gl and the IOP value was confirmed (p < 0.05). In this group, the positive correlations between the TGF beta1 concentration in the aqueous humor and the presence of pseudoexfoliation (p < 0.01), on the one hand, and between the TIMP-2 level and the presence of pseudoexfoliation (p < 0.05), on the other, were reported. A statistically significant positive correlation of TGF-beta1 and MMP-2, and the degree of chamber angle pigmentation in the PEX Gl group was confirmed (p < 0.05). In the POAG group, TIMP-2 values were in a negative correlation with the degree of pigmentation (p < 0.05), and the IOP value (p < 0.05).

CONCLUSION:

TGF beta1 and MMP-2 affect the degree of chamber angle pigmentation and the degree of pseudoexfoliation in patients with pseudoexfoliative glaucoma.

• Authors: Jasmina Djordjević-Jocić, Gordana Zlatanović, Dragan Veselinović, Predrag Jovanović, Vidosava Djordjević, Lilika Zvezdanović, Gordana Stanković-Babić, Milena Vujanović, Sonja Cekić, Matthias Zenkel, Ursula Schlotzer-Schrehardt
• Journal: Vojnosanitetski pregled (Vojnosanit Pregl) Vol. 69 Issue 3 Pg. 231-6 (Mar 2012) ISSN: 0042-8450 [Print] Serbia
• PMID: 22624408 (Publication Type: Journal Article)

Chemical References

• TIMP2 protein, human
• Transforming Growth Factor beta1
• Tissue Inhibitor of Metalloproteinase-2
• Matrix Metalloproteinase 2

Topics

• Aged
• Aqueous Humor (metabolism)
• Cataract (metabolism)
• Exfoliation Syndrome (metabolism, pathology)
• Glaucoma, Open-Angle (metabolism)
• Humans
• Matrix Metalloproteinase 2 (metabolism)
• Middle Aged
• Tissue Inhibitor of Metalloproteinase-2 (metabolism)
• Transforming Growth Factor beta1 (metabolism)