Dioxinodehydroeckol inhibits melanin synthesis through PI3K/Akt signalling pathway in α-melanocyte-stimulating hormone-treated B16F10 cells.

Abstract

Antimelanogenic activity has previously been reported in ethyl acetate fraction of Ecklonia stolonifera. In this study, using the isolated dioxinodehydroeckol from the fraction, we sought to investigate an antimelanogenic signalling pathway in α-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells. Treatment with dioxinodehydroeckol inhibited the cellular melanin contents and expression of melanogenesis-related proteins, including microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related proteins TRP-1 and TRP-2. Moreover, dioxinodehydroeckol stimulated phosphorylation of Akt in a dose-dependent manner without affecting phosphorylation of ERK. These data suggest that dioxinodehydroeckol reduces melanin synthesis through the MITF regulation dependent upon PI3K/Akt signalling pathway.

• Authors: Min-Sup Lee, Ho-Dong Yoon, Jae-Il Kim, Jae-Sue Choi, Dae-Seok Byun, Hyeung-Rak Kim
• Journal: Experimental dermatology (Exp Dermatol) Vol. 21 Issue 6 Pg. 471-3 (Jun 2012) ISSN: 1600-0625 [Electronic] Denmark
• PMID: 22621193 (Publication Type: Letter, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 John Wiley & Sons A/S.

Chemical References

• Dioxins
• Melanins
• dioxinodehydroeckol
• alpha-MSH
• Phosphatidylinositol 3-Kinases
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Cell Line, Tumor
• Dioxins (isolation & purification, pharmacology)
• Melanins (antagonists & inhibitors, biosynthesis)
• Mice
• Phaeophyta (chemistry)
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• alpha-MSH