Defluorination of sodium fluoroacetate by bacteria from soil and plants in Brazil.

Abstract

The aim of this work was to isolate and identify bacteria able to degrade sodium fluoroacetate from soil and plant samples collected in areas where the fluoroacetate-containing plants Mascagnia rigida and Palicourea aenofusca are found. The samples were cultivated in mineral medium added with 20 mmol L(-1) sodium fluoroacetate. Seven isolates were identified by 16S rRNA gene sequencing as Paenibacillus sp. (ECPB01), Burkholderia sp. (ECPB02), Cupriavidus sp. (ECPB03), Staphylococcus sp. (ECPB04), Ancylobacter sp. (ECPB05), Ralstonia sp. (ECPB06), and Stenotrophomonas sp. (ECPB07). All seven isolates degraded sodium-fluoroacetate-containing in the medium, reaching defluorination rate of fluoride ion of 20 mmol L(-1). Six of them are reported for the first time as able to degrade sodium fluoroacetate (SF). In the future, some of these microorganisms can be used to establish in the rumen an engineered bacterial population able to degrade sodium fluoroacetate and protect ruminants from the poisoning by this compound.

• Authors: Expedito K A Camboim, Michelle Z Tadra-Sfeir, Emanuel M de Souza, Fabio de O Pedrosa, Paulo P Andrade, Chris S McSweeney, Franklin Riet-Correa, Marcia A Melo
• Journal: TheScientificWorldJournal (ScientificWorldJournal) Vol. 2012 Pg. 149893 ( 2012) ISSN: 1537-744X [Electronic] United States
• PMID: 22619595 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluoroacetates
• RNA, Ribosomal, 16S
• Fluorine
• fluoroacetic acid

Topics

• Animals
• Bacteria (classification, genetics, metabolism)
• Biodegradation, Environmental
• Brazil
• Cattle
• Fluorine (metabolism)
• Fluoroacetates (metabolism, poisoning)
• Phylogeny
• Plants (microbiology)
• Poisoning (mortality, prevention & control, veterinary)
• RNA, Ribosomal, 16S (genetics)
• Soil Microbiology