Assessing the potential clinical utility of transplantations of neural and mesenchymal stem cells for treating neurodegenerative diseases.

Abstract

Treatments for neurodegenerative diseases have little impact on the long-term patient health. However, cellular transplants of neuroblasts derived from the aborted embryonic brain tissue in animal models of neurodegenerative disorders and in patients have demonstrated survival and functionality in the brain. However, ethical and functional problems due to the use of this fetal tissue stopped most of the clinical trials. Therefore, new cell sources were needed, and scientists focused on neural (NSCs) and mesenchymal stem cells (MSCs). When transplanted in the brain of animals with Parkinson's or Huntington's disease, NSCs and MSCs were able to induce partial functional recovery by promoting neuroprotection and immunomodulation. MSCs are more readily accessible than NSCs due to sources such as the bone marrow. However, MSCs are not capable of differentiating into neurons in vivo where NSCs are. Thus, transplantation of NSCs and MSCs is interesting for brain regenerative medicine. In this chapter, we detail the methods for NSCs and MSCs isolation as well as the transplantation procedures used to treat rodent models of neurodegenerative damage.

• Authors: Laurent Lescaudron, C Boyer, Virginie Bonnamain, K D Fink, X Lévêque, J Rossignol, V Nerrière-Daguin, A C Malouet, F Lelan, N D Dey, D Michel-Monigadon, M Lu, I Neveu, S von Hörsten, P Naveilhan, G L Dunbar
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 879 Pg. 147-64 ( 2012) ISSN: 1940-6029 [Electronic] United States
• PMID: 22610559 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Cell Separation (methods)
• Cells, Cultured
• Mesenchymal Stem Cells (cytology)
• Neural Stem Cells (cytology)
• Neurodegenerative Diseases (therapy)
• Rats
• Rats, Sprague-Dawley
• Stem Cell Transplantation (methods)