Electromagnetic field stimulation potentiates endogenous myelin repair by recruiting subventricular neural stem cells in an experimental model of white matter demyelination.

Abstract

Electromagnetic fields (EMFs) may affect the endogenous neural stem cells within the brain. The aim of this study was to assess the effects of EMFs on the process of toxin-induced demyelination and subsequent remyelination. Demyelination was induced using local injection of lysophosphatidylcholine within the corpus callosum of adult female Sprague-Dawley rats. EMFs (60 Hz; 0.7 mT) were applied for 2 h twice a day for 7, 14, or 28 days postlesion. BrdU labeling and immunostaining against nestin, myelin basic protein (MBP), and BrdU were used for assessing the amount of neural stem cells within the tissue, remyelination patterns, and tracing of proliferating cells, respectively. EMFs significantly reduced the extent of demyelinated area and increased the level of MBP staining within the lesion area on days 14 and 28 postlesion. EMFs also increased the number of BrdU- and nestin-positive cells within the area between SVZ and lesion as observed on days 7 and 14 postlesion. It seems that EMF potentiates proliferation and migration of neural stem cells and enhances the repair of myelin in the context of demyelinating conditions.

• Authors: Mohammad Amin Sherafat, Motahareh Heibatollahi, Somayeh Mongabadi, Fatemeh Moradi, Mohammad Javan, Abolhassan Ahmadiani
• Journal: Journal of molecular neuroscience : MN (J Mol Neurosci) Vol. 48 Issue 1 Pg. 144-53 (Sep 2012) ISSN: 1559-1166 [Electronic] United States
• PMID: 22588976 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intermediate Filament Proteins
• Myelin Basic Protein
• Nerve Tissue Proteins
• Nes protein, rat
• Nestin
• Bromodeoxyuridine

Topics

• Animals
• Bromodeoxyuridine (metabolism)
• Cell Movement (physiology, radiation effects)
• Cell Proliferation (radiation effects)
• Corpus Callosum (physiology, radiation effects)
• Disease Models, Animal
• Electric Stimulation Therapy (methods)
• Female
• Intermediate Filament Proteins (metabolism)
• Multiple Sclerosis (physiopathology, therapy)
• Myelin Basic Protein (metabolism)
• Myelin Sheath (metabolism, radiation effects)
• Nerve Degeneration (physiopathology, therapy)
• Nerve Regeneration (physiology, radiation effects)
• Nerve Tissue Proteins (metabolism)
• Nestin
• Neural Stem Cells (cytology, radiation effects)
• Rats
• Rats, Sprague-Dawley
• Stem Cell Niche (physiology)
• Transcranial Magnetic Stimulation (methods)