Efficacy of Fungiflora Y staining for the diagnosis of oral erythematous candidiasis.

Abstract

OBJECTIVE:

The purpose of this study was to investigate the efficacy of Fungiflora Y staining (fluorescent stain) for the diagnosis of erythematous candidiasis.

SUBJECTS AND METHODS:

This study enrolled 48 patients who were diagnosed with erythematous candidiasis and who underwent fungal culture and microscopic examination of a smear specimen stained with CytoQuick (modification of the Giemsa stain) and Fungiflora Y. Fungiflora Y staining was observed using a portable fluorescent microscope (CyScope(®)). The sensitivity, specificity and positive and negative predictive values were calculated using fungal culture as the gold standard test. Accuracy was calculated, and the difference between the CytoQuick and Fungiflora Y groups was examined using contingency tables and the chi-square test.

RESULTS:

The sensitivity and specificity of the CytoQuick stain was 0.51 and 0.91, respectively; the positive predictive value was 0.95, and the negative predictive value was 0.36. The sensitivity and specificity of the Fungiflora Y stain was 0.84 and 1.0, respectively; the positive predictive value was 1.00, and the negative predictive value was 0.65. The accuracy of Fungiflora Y (0.88) was superior to that of CytoQuick (0.60) (p = 0.0052).

CONCLUSIONS:

Microscopic examinations of smear specimens using a combination of Fungiflora Y staining and the CyScope(®) portable fluorescent microscope was found to be useful for the diagnosis of oral erythematous candidiasis.

• Authors: Mariko R Okamoto, Miho Kamoi, Shigeo Yamachika, Akihisa Tsurumoto, Takahiro Imamura, Ken Yamamoto, Shinichi Kadomatsu, Ichiro Saito, Nobuko Maeda, Yoichi Nakagawa
• Journal: Gerodontology (Gerodontology) Vol. 30 Issue 3 Pg. 220-5 (Sep 2013) ISSN: 1741-2358 [Electronic] England
• PMID: 22582792 (Publication Type: Comparative Study, Journal Article)
• Copyright: © 2012 John Wiley & Sons A/S and The Gerodontology Society. Published by John Wiley & Sons Ltd.

Chemical References

• Azure Stains
• Coloring Agents
• Fluorescent Dyes
• Fungiflora Y
• Organic Chemicals

Topics

• Adult
• Aged
• Aged, 80 and over
• Azure Stains
• Candida albicans (isolation & purification)
• Candida glabrata (isolation & purification)
• Candida tropicalis (isolation & purification)
• Candidiasis, Oral (diagnosis)
• Coloring Agents
• Cytodiagnosis (methods)
• Female
• Fluorescent Dyes
• Humans
• Male
• Microscopy, Fluorescence (methods)
• Middle Aged
• Mycology (methods)
• Organic Chemicals
• Predictive Value of Tests
• Sensitivity and Specificity
• Tongue Diseases (microbiology)