Antiproliferative effect of catechin in GRX cells.

Abstract

The phenolic compounds present in cocoa seeds have been studied regarding health benefits, such as antioxidant and anti-inflammatory activities. Fibrosis is a wound healing response that occurs in almost all patients with chronic liver injury. A large number of cytokines and soluble intercellular mediators are related to changes in the behavior and phenotype of the hepatic stellate cell (HSC) that develop a fibrogenic and contractile phenotype leading to the development of fibrosis. The objective of this study was to assess the catechin effect in GRX liver cells in activities such as cell growth and inflammation. The GRX cells treatment with catechin induced a significant decrease in cell growth. This mechanism does not occur by apoptosis or even by autophagy because there were no alterations in expression of caspase 3 and PARP (apoptosis), and LC3 (autophagy). The expression of p27 and p53 proteins, regulators of the cell cycle, showed increased expression, while COX-2 and IL-6 mRNA showed a significant decrease in expression. This study shows that catechin decreases cell growth in GRX cells and, probably, this decrease does not occur by apoptosis or autophagy but through an anti-inflammatory effect and cell cycle arrest. Catechin also significantly decreased the production of TGF-β by GRX cells, showing a significant antifibrotic effect.

• Authors: Cristina Machado Bragança de Moraes, Denizar Alberto da Silva Melo, Roberto Christ Vianna Santos, Shanna Bitencourt, Fernanda Cristina Mesquita, Fernanda dos Santos de Oliveira, Edgardo Rodriguez-Carballo, Ramon Bartrons, Jose Luis Rosa, Francesc Pujol Ventura, Jarbas Rodrigues de Oliveira
• Journal: Biochemistry and cell biology = Biochimie et biologie cellulaire (Biochem Cell Biol) Vol. 90 Issue 4 Pg. 575-84 (Aug 2012) ISSN: 1208-6002 [Electronic] Canada
• PMID: 22574829 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-6
• Transforming Growth Factor beta
• Tumor Suppressor Protein p53
• Catechin
• Caspase 3

Topics

• Autophagy
• Cacao (chemistry)
• Caspase 3 (metabolism)
• Catechin (pharmacology)
• Cell Proliferation
• Cells, Cultured
• Hepatic Stellate Cells (cytology, drug effects, metabolism)
• Humans
• Interleukin-6 (metabolism)
• Liver (cytology, metabolism)
• Transforming Growth Factor beta (metabolism)
• Tumor Suppressor Protein p53 (metabolism)