Abstract |
In this study, 714 cows from 26 dairy herds were reclassified as healthy or mastitic cows on the basis of long-term somatic cell count (SCC) in milk. Cows with more than three consecutive lactation records of SCC from the first or second to fifth lactation, were selected, and their BoLA-DRB3 (DRB3) alleles were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Cows with an SCC of < 200 000 cells/mL in all monthly records were classified as healthy (n=91). Cows with an SCC of > 300 000 cells/mL in two consecutive tests or four non-consecutive tests or cows with an SCC of > 500 000 cells/mL in any one test during lactation, regardless of parity, were classified as mastitic (n=201). Mastitic cows (n=153) from another 40 herds were considered to be infected if bacteriological testing revealed mastitis pathogens in milk. Their DRB3 alleles were identified using PCR-sequence-based typing (PCR-SBT). The differences in DRB3 allelic frequencies between healthy cows and cows with various degrees of mastitis were re-investigated. Moreover, the associations of various amino acid motifs in DRB3 alleles with resistance or susceptibility to mastitis pathogens were re-examined. DRB3.2*8(DRB3*1201) and DRB3.2*16(DRB3*1501) alleles were found to be associated with susceptibility, while DRB3.2*22(DRB3*1101), DRB3.2*23(DRB3*2703), and DRB3.2*24(DRB3*0101) alleles were found to be associated with resistance.
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Authors | Tatsuyuki Yoshida, Hiroki Furuta, Yasuko Kondo, Harutaka Mukoyama |
Journal | Animal science journal = Nihon chikusan Gakkaiho
(Anim Sci J)
Vol. 83
Issue 5
Pg. 359-66
(May 2012)
ISSN: 1740-0929 [Electronic] Australia |
PMID | 22574787
(Publication Type: Journal Article)
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Copyright | © 2011 The Authors. Animal Science Journal © 2011 Japanese Society of Animal Science. |
Chemical References |
- BoLA-DRB3 antigen
- Histocompatibility Antigens Class II
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Topics |
- Alleles
- Animals
- Cattle
- Disease Resistance
(genetics)
- Female
- Genetic Predisposition to Disease
(genetics)
- Histocompatibility Antigens Class II
(genetics)
- Japan
- Mastitis, Bovine
(genetics)
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
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