[Effects of shikonin on the proliferation and apoptosis of HL-60 cells].

Abstract

OBJECTIVE:

To explore the mechanism of shikonin for inducing the apoptosis of human promyelocytic leukemia cell HL-60.

METHODS:

The effects of shikonin on the HL-60 cell proliferation were detected using MTT. The apoptosis rate was analyzed by Annexin-V/PI double staining. The expression level of the bcl-2 gene was detected using semi-quantitative reverse transcriptase PCR (RT-PCR), thus analyzing the correlation between the bcl-2 expression level and the apoptosis of HL-60.

RESULTS:

Shikonin could inhibit the proliferation of HL-60 cells with the concentration range of 1-8 microg/mL in a time- and concentration-dependent manner. Two microg/mL shikonin could induce the apoptosis of HL-60 cells in a time-dependent manner. The expression level of bcl-2 was obviously down-regulated at 2 microg/mL shikonin.

CONCLUSIONS:

Shikonin could induce the apoptosis of HL-60 cells. Its mechanism was correlated with down-regulation of the expression level of bcl-2.

• Authors: Zhi-Lu Chen, Qi-Zhou Dai, Yong Wang
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 32 Issue 2 Pg. 239-43 (Feb 2012) ISSN: 1003-5370 [Print] China
• PMID: 22574601 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Naphthoquinones
• Proto-Oncogene Proteins c-bcl-2
• shikonin

Topics

• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• HL-60 Cells
• Humans
• Naphthoquinones (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)