BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells.

Abstract

DEC1 (BHLHE40/Stra13/Sharp2) and DEC2 (BHLHE41/Sharp1) are basic helix-loop-helix (bHLH) transcription factors that are involved in the regulation of apoptosis, cell proliferation, circadian rhythms and the response to hypoxia. We previously showed the functional effects of DEC1 and DEC2 on apoptosis in human breast cancer MCF-7 cells. However, the roles of DEC1 and DEC2 in oral cancer are poorly understood. We examined whether DEC1 and DEC2 are involved in the regulation of apoptosis in human oral cancer HSC-3 and CA9-22 cells. The expression of DEC2 was upregulated by cis-diamminedichloroplatinum (II) (cisplatin: CDDP) treatment in HSC-3 cells, whereas CDDP treatment had little effects on the expression of DEC2 in CA9-22 cells. We showed that DEC2 overexpression inhibits pro-apoptotic factor Bim and inhibits apoptosis induced by CDDP in HSC-3 cells, whereas it had little effects on apoptosis in CA9-22 cells. DEC1 overexpression had little effects on apoptosis induced by CDDP in these cells. We also found that CDDP upregulated the amounts of DEC2 in the nucleus in HSC-3 cells. These results suggest that DEC2 has anti-apoptotic effects on apoptosis induced by CDDP in HSC-3 cells.

• Authors: Yunyan Wu, Fuyuki Sato, Ujjal Kumar Bhawal, Takeshi Kawamoto, Katsumi Fujimoto, Mitsuhide Noshiro, Hiroko Seino, Satoko Morohashi, Yukio Kato, Hiroshi Kijima
• Journal: Biomedical research (Tokyo, Japan) (Biomed Res) Vol. 33 Issue 2 Pg. 75-82 (Apr 2012) ISSN: 1880-313X [Electronic] Japan
• PMID: 22572381 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Apoptosis Regulatory Proteins
• BCL2L11 protein, human
• BHLHE41 protein, human
• Basic Helix-Loop-Helix Transcription Factors
• Bcl-2-Like Protein 11
• DELEC1 protein, human
• Membrane Proteins
• Proto-Oncogene Proteins
• Tumor Suppressor Proteins
• PARP1 protein, human
• Poly (ADP-Ribose) Polymerase-1
• Poly(ADP-ribose) Polymerases
• Caspases
• Cisplatin

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Basic Helix-Loop-Helix Transcription Factors (genetics, metabolism, physiology)
• Bcl-2-Like Protein 11
• Caspases (metabolism)
• Cell Line, Tumor
• Cell Nucleus (metabolism)
• Cell Survival (drug effects)
• Cisplatin (pharmacology)
• Gene Expression (drug effects)
• Humans
• Membrane Proteins (genetics, metabolism)
• Mouth Neoplasms
• Poly (ADP-Ribose) Polymerase-1
• Poly(ADP-ribose) Polymerases (metabolism)
• Proto-Oncogene Proteins (genetics, metabolism)
• Tumor Suppressor Proteins (physiology)
• Up-Regulation (drug effects)