Abstract | BACKGROUND: METHODS: In total, 104 resected lung adenocarcinomas from women who were nonsmokers were analyzed concurrently for EGFR mutations, ALK rearrangements, and mRNA expression of the ERCC1, RRM1, TS, and BRCA1 genes. EGFR mutations were detected with a proprietary detection kit, ALK rearrangements were detected by polymerase chain reaction analysis, and genetic mRNA expression was detected by real-time polymerase chain reaction analysis. RESULTS: Of 104 patients, 73 (70.2%) had EGFR mutations, and 10 (9.6%) had ALK rearrangements. ERCC1 mRNA levels in patients who had EGFR mutations were 3.44 ± 1.94 × 10(-3) , which were significantly lower than the levels in patients who were positive for ALK rearrangements and in patients who were negative for both biomarkers (4.60 ± 1.95 × 10(-3) and 4.95 ± 2.33 × 10(-3) , respectively; P = .010). However, TS mRNA levels were significantly lower in patients who had EGFR mutations (1.15 ± 1.38 × 10(-3) vs 2.69 ± 3.97 × 10(-3) ; P = .006) or ALK rearrangements (1.21 ± 0.78 × 10(-3) vs 2.69 ± 3.97 × 10(-3) ; P = .020) than in patients who were negative for both biomarkers. CONCLUSIONS: NSCLC specimens that harbored activating EGFR mutations were more likely to express low ERCC1 and TS mRNA levels, whereas patients with NSCLC who had ALK rearrangement were more likely to express low TS mRNA levels.
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Authors | Shengxiang Ren, Xiaoxia Chen, Peng Kuang, Limou Zheng, Chunxia Su, Jiayu Li, Bing Li, Yongshen Wang, Lu Liu, Qiong Hu, Jie Zhang, Liang Tang, Xuefei Li, Caicun Zhou, Gerald Schmid-Bindert |
Journal | Cancer
(Cancer)
Vol. 118
Issue 22
Pg. 5588-94
(Nov 15 2012)
ISSN: 1097-0142 [Electronic] United States |
PMID | 22569898
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 American Cancer Society. |
Chemical References |
- BRCA1 Protein
- BRCA1 protein, human
- DNA-Binding Proteins
- RNA, Messenger
- Tumor Suppressor Proteins
- DNA
- RRM1 protein, human
- Ribonucleoside Diphosphate Reductase
- Thymidylate Synthase
- ALK protein, human
- Anaplastic Lymphoma Kinase
- EGFR protein, human
- ErbB Receptors
- Receptor Protein-Tyrosine Kinases
- ERCC1 protein, human
- Endonucleases
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Topics |
- Adenocarcinoma
(genetics)
- Adult
- Aged
- Anaplastic Lymphoma Kinase
- BRCA1 Protein
(genetics)
- Carcinoma, Non-Small-Cell Lung
(genetics)
- DNA
(biosynthesis)
- DNA Repair
(genetics)
- DNA Replication
(genetics)
- DNA-Binding Proteins
(genetics)
- Endonucleases
(genetics)
- ErbB Receptors
(genetics)
- Female
- Gene Rearrangement
- Humans
- Lung Neoplasms
(genetics)
- Middle Aged
- Mutation
- RNA, Messenger
(biosynthesis)
- Receptor Protein-Tyrosine Kinases
(genetics)
- Ribonucleoside Diphosphate Reductase
- Smoking
- Thymidylate Synthase
(genetics)
- Tumor Suppressor Proteins
(genetics)
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