Stem cell-based photodynamic therapy.

Abstract	We have transfected murine neural stem cells (NSCs) and rat umbilical cord matrix-derived stem cells (RUCMSCs) with a plasmid expressing gaussia luciferase (gLuc). These cells are engineered to secrete the luciferase. We have used gLuc containing supernatant from culturing the NSCs to perform in vitro photodynamic therapy of murine melanoma cells (B16F10), and RUCMSCs to perform in vivo PDT of lung melanomas in C57BL/6 mice. The treatment system was comprised of aminolevulic acid as a prodrug for the synthesis of the photosensitizer protoporphyrin IX, gaussia luciferase, and its' substrate coelenterazine. A significant reduction of the number of live melanoma cells in vitro and a borderline significant retardation of tumour growth in vivo was observed after coelenterazine-mediated PDT.
Authors	Tej B Shrestha, Gwi M Seo, Matthew T Basel, Mausam Kalita, Hongwang Wang, David Villanueva, Marla Pyle, Sivasai Balivada, Raja Shekar Rachakatla, Heather Shinogle, Prem S Thapa, David Moore, Deryl L Troyer, Stefan H Bossmann
Journal	Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (Photochem Photobiol Sci) Vol. 11 Issue 7 Pg. 1251-8 (Jul 2012) ISSN: 1474-9092 [Electronic] England
PMID	22565929 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References	Imidazoles Photosensitizing Agents Protoporphyrins Pyrazines coelenterazine Aminolevulinic Acid protoporphyrin IX Luciferases
Topics	Aminolevulinic Acid (chemistry, pharmacology, therapeutic use) Animals Cell Line, Tumor Cell Survival (drug effects) Female Fetal Blood (cytology) Imidazoles (chemistry, pharmacology) Luciferases (genetics, metabolism) Lung Neoplasms (drug therapy) Melanoma (drug therapy) Mice Mice, Inbred C57BL Neural Stem Cells (cytology, metabolism) Oxidation-Reduction Photochemotherapy Photosensitizing Agents (chemistry, therapeutic use, toxicity) Plasmids (metabolism) Protoporphyrins (biosynthesis, therapeutic use, toxicity) Pyrazines (chemistry, pharmacology) Rats Stem Cell Transplantation Stem Cells (cytology, metabolism) Transfection

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