Mouse model for efficacy testing of antituberculosis agents via intrapulmonary delivery.

Abstract

Here we describe an experimental murine model that allows for aerosolized antituberculosis drug efficacy testing. Intrapulmonary aerosol delivery of isoniazid, capreomycin, and amikacin to mice with pulmonary infection of Mycobacterium tuberculosis demonstrated efficacy in reducing pulmonary bacterial loads similar to that seen by standard drug delivery methods, even when lower concentrations of drugs and fewer doses were used in the aerosolized drug regimens. Interestingly, intrapulmonary delivery of isoniazid also reduced the bacterial load in the spleen.

• Authors: Mercedes Gonzalez-Juarrero, Lisa K Woolhiser, Elizabeth Brooks, Mary Ann DeGroote, Anne J Lenaerts
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 56 Issue 7 Pg. 3957-9 (Jul 2012) ISSN: 1098-6596 [Electronic] United States
• PMID: 22547626 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antitubercular Agents
• Isoniazid

Topics

• Administration, Inhalation
• Animals
• Antitubercular Agents (administration & dosage, therapeutic use)
• Female
• Isoniazid (administration & dosage, therapeutic use)
• Lung (metabolism, microbiology)
• Mice
• Mice, Inbred BALB C
• Mycobacterium tuberculosis (drug effects, pathogenicity)
• Tuberculosis, Pulmonary (drug therapy)