Abstract | BACKGROUND: METHODS: MTT assay was used to determine IC(50) values in BGC-823, MKN-28, LOVO and SW-116 cells after gambogic acid and docetaxel administration. Median effect analysis was applied for determination of synergism and antagonism. Synergistic interaction between gambogic acid and docetaxel was evaluated using the combination index (CI) method. Furthermore, cellular apoptosis was analyzed by Annexin-V and propidium iodide (PI) double staining. Additionally, mRNA expression of drug-associated genes, i.e., β-tublin III and tau, and the apoptosis-related gene survivin, were measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). RESULTS: CONCLUSIONS:
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Authors | Zhengyun Zou, Li Xie, Jia Wei, Lixia Yu, Xiaoping Qian, Junhao Chen, Tingting Wang, Baorui Liu |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 12
Pg. 58
(Apr 30 2012)
ISSN: 1472-6882 [Electronic] England |
PMID | 22546220
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- BIRC5 protein, human
- Inhibitor of Apoptosis Proteins
- Plant Extracts
- RNA, Messenger
- Survivin
- TUBB3 protein, human
- Taxoids
- Tubulin
- Xanthones
- tau Proteins
- Docetaxel
- gambogic acid
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Topics |
- Antineoplastic Agents
(administration & dosage, pharmacology, therapeutic use)
- Antineoplastic Combined Chemotherapy Protocols
(pharmacology, therapeutic use)
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Colorectal Neoplasms
(drug therapy, genetics, metabolism)
- Docetaxel
- Drug Synergism
- Herb-Drug Interactions
- Humans
- Inhibitor of Apoptosis Proteins
(genetics, metabolism)
- Inhibitory Concentration 50
- Phytotherapy
- Plant Extracts
(pharmacology, therapeutic use)
- RNA, Messenger
(metabolism)
- Stomach Neoplasms
(drug therapy, genetics, metabolism)
- Survivin
- Taxoids
(administration & dosage, pharmacology, therapeutic use)
- Tubulin
(genetics, metabolism)
- Xanthones
(administration & dosage, pharmacology, therapeutic use)
- tau Proteins
(genetics, metabolism)
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