This study was aimed to investigate the pro coagulation effects of
hemocoagulase atrix and its effective components (
batroxobin and
factor X activator) on plasma of normal subjects and patients with
bleeding disorders and their mechanisms. Activated partial thromboplastin time (APTT) and prothrombin time (PT) were measured. The factor (F)X activation and
thrombin generation were analyzed by using
chromogenic substrate method. The results showed that the plasma APTT of normal subjects was shortened by
hemocoagulase atrix,
batroxobin and FX activator, and the effect of FX activator was found to be concentration-dependent (r = 0.889, P < 0.05). The prolonged APTT of plasma from patients with
bleeding disorders could be corrected by
hemocoagulase atrix,
batroxobin and FX activator, but PT showed no great changes resulted from the treatments. FX activator could promote FX activation and
thrombin generation, while neither
hemocoagulase atrix nor
batroxobin showed such abilities. It is concluded that
hemocoagulase atrix promotes coagulation process, and corrects coagulation abnormalities in patients with
bleeding disorders, its main component
batroxobin directly acts on
fibrinogen, and FX activator promotes
thrombin generation through activating FX.