Comparison of near-infrared fluorescent deoxyglucose probes with different dyes for tumor diagnosis in vivo.

Abstract

Glucose plays a central role in the cellular energy metabolism. Malignant tumors exhibit an elevated rate of glycolysis over normal tissues. In this study, two near-infrared fluorescent dyes, Cypate and ICG-Der-02, with different water solubility, were conjugated to 2-amino-2-deoxy-D-glucose (2DG) to form Cypate-2DG and ICG-Der-02-2DG, respectively, for NIR fluorescent imaging of tumors in nude mice. The clear routes and tumor targeting abilities of the two NIR fluorescent 2DG probes were compared. Results showed that ICG-Der-02-2DG with higher hydrophilicity was cleared faster by kidneys than the more lipophilic Cypate-2DG. Cypate-2DG had slower but stronger tumor targeting ability compared with ICG-Der-02-2DG. To investigate the correlation between the targeting ability of the probe and the glucose transporter (GLUT1) expression levels of cancer cells, the accumulation of Cypate-2DG in tumors was assessed in MCF-7/estradiol, U87MG, MCF-7 and MDA-MB-435 tumor xenografts, which express different levels of GLUT1. The results show that both Cypate-2DG and ICG-Der-02-2DG possess tumor targeting ability on all the tumors examined, with a proportional correlation to GLUT1 expression. The findings demonstrate the broad applicability of these molecular probes for optical imaging of tumors and glucose-related pathologies.

• Authors: Jing Guo, Changli Du, Lingling Shan, Hongyan Zhu, Bing Xue, Zhiyu Qian, Samuel Achilefu, Yueqing Gu
• Journal: Contrast media & molecular imaging (Contrast Media Mol Imaging) 2012 May-Jun Vol. 7 Issue 3 Pg. 289-301 ISSN: 1555-4317 [Electronic] England
• PMID: 22539399 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 John Wiley & Sons, Ltd.

Chemical References

• Carbocyanines
• Coloring Agents
• Fluorescent Dyes
• Glucose Transporter Type 1
• ICG-Der-02-2DG
• cypate-2DG
• Deoxyglucose
• Glucosamine

Topics

• Animals
• Carbocyanines (chemical synthesis, pharmacokinetics)
• Coloring Agents
• Deoxyglucose (chemistry)
• Female
• Fluorescent Dyes (chemistry)
• Glucosamine (analogs & derivatives, chemical synthesis, pharmacokinetics)
• Glucose Transporter Type 1 (metabolism)
• Humans
• Mammary Neoplasms, Experimental (diagnosis, metabolism)
• Mice
• Mice, Nude
• Spectroscopy, Near-Infrared
• Tissue Distribution
• Transplantation, Heterologous
• Tumor Cells, Cultured