The role of N-acetylglucosaminyltransferases V in the malignancy of human hepatocellular carcinoma.

Abstract

To investigate the role of N-acetylglucosaminyltransferases V (GnT-V) in the malignancy of human hepatocellular carcinoma (HCC), the GnT-V stably suppressed cell line HepG2 GnT-V/1564 was constructed from HepG2. The proliferation, migration, invasion, metastasis of HepG2 GnT-V/1564 was investigated both in vitro and in vivo. The clinical pathological significance of GnT-V expression was also studied in 140 cases of HCC tissues. This study showed that down-regulation of GnT-V inhibited the proliferation, migration and invasion of the HepG2 cells. In addition, GnT-V expression was shown in 138 cases of 140 (98.6%) HCC samples, in 3 cases of 31 (9.7%) in liver cirrhosis cases and in 1 cases of 20 (5.0%) in normal liver tissues. Besides, a higher level of GnT-V was observed more frequently in the advanced tumors with higher T stage and histological grade. These data suggested that GnT-V expression was positively related with malignancy in HCC and GnT-V may be both a differentiation marker and a potential target for the treatment of HCC.

• Authors: Ting Wei, Qiulian Liu, Fuli He, Weiliang Zhu, Lijuan Hu, Linlang Guo, Jian Zhang
• Journal: Experimental and molecular pathology (Exp Mol Pathol) Vol. 93 Issue 1 Pg. 8-17 (Aug 2012) ISSN: 1096-0945 [Electronic] Netherlands
• PMID: 22537550 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• N-Acetylglucosaminyltransferases
• alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase

Topics

• Animals
• Carcinoma, Hepatocellular (enzymology, pathology)
• Cell Movement
• Cell Proliferation
• Down-Regulation
• Hep G2 Cells
• Humans
• Liver (enzymology)
• Liver Cirrhosis (enzymology)
• Liver Neoplasms (enzymology, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• N-Acetylglucosaminyltransferases (biosynthesis)
• Neoplasm Grading
• Neoplasm Invasiveness
• Neoplasm Staging
• Xenograft Model Antitumor Assays