Recombinant hepatitis B virus surface antigen formulated with B-type CpG oligodeoxynucleotide induces therapeutic immunity against hepatitis B virus surface antigen-expressing liver cancer cells in mice.

Abstract	To develop a therapeutic vaccine against hepatitis B virus surface antigen (HBsAg)-expressing liver cancer, we tried to prepare a vaccine by formulating recombinant HBsAg with BW006, a B type CpG oligodeoxynucleotide (ODN) with Th1-biasing activity, and examined its potency of inducing therapeutic immunity against HBsAg-expressing liver cancer cells in mice. When applied therapeutically, BW006 could assist HBsAg to induce vigorous immune responses capable of inhibiting the growth of HBsAg-expressing liver cancer cells and prolonging the survival of mice bearing HBsAg-expressing liver cancer cells. In vivo and in vitro experiments showed that the BW006-adjuvanted HBsAg enhanced the production of IgG2a antibodies, interferon-γ, and interleukin-12 and facilitated the generation of specific cytotoxic T lymphocyte that killed the HBsAg-expressing liver cancer cells. These results suggest that the BW006-adjuvanted HBsAg might be developed into a candidate tumor vaccine for the treatment of HBsAg-expressing liver cancer.
Authors	Xiaojing Zhou, Hongfei Wei, Peng Sun, Xiuli Wu, Min Wan, Peng Zhang, Sheng Guo, Tiesuo Zhao, Yongli Yu, Liying Wang
Journal	Cancer biotherapy & radiopharmaceuticals (Cancer Biother Radiopharm) Vol. 27 Issue 4 Pg. 234-42 (May 2012) ISSN: 1557-8852 [Electronic] United States
PMID	22537404 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antigens, CD Antigens, Differentiation, T-Lymphocyte CD69 antigen Cancer Vaccines Hepatitis B Surface Antigens Immunoglobulin G Lectins, C-Type Oligodeoxyribonucleotides Recombinant Proteins Interleukin-12 Interferon-gamma
Topics	Animals Antigens, CD (metabolism) Antigens, Differentiation, T-Lymphocyte (metabolism) Cancer Vaccines (immunology, therapeutic use) Carcinoma, Hepatocellular (drug therapy, immunology) Cell Proliferation Female Hepatitis B Surface Antigens (immunology) Humans Immunoglobulin G (metabolism) Interferon-gamma (metabolism) Interleukin-12 (metabolism) Kaplan-Meier Estimate Lectins, C-Type (metabolism) Liver Neoplasms (drug therapy, immunology) Mice Mice, Inbred BALB C Neoplasm Transplantation Oligodeoxyribonucleotides (immunology) Recombinant Proteins T-Lymphocytes, Cytotoxic Tumor Cells, Cultured

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