Monitoring microcirculation changes in port wine stains during vascular targeted photodynamic therapy by laser speckle imaging.

Abstract

This study was conducted to test laser speckle perfusion imaging (LSPI) for imaging microcirculation and monitoring microcirculatory changes of port wine stains (PWS) during vascular targeted photodynamic therapy (V-PDT). Before and 5 min after V-PDT, PWS lesions and the corresponding contralateral healthy skins of 24 PWS patients were scanned, whereas seven PWS patients were scanned throughout V-PDT. V-PDT was conducted immediately after intravenous injection of photocarcinorin (4-5 mg kg(-1)). A 532 nm laser was used for irradiation (power density: 80-100 mW cm(-2), exposure time: 20-50 min). Before V-PDT, all 24 PWS patients demonstrated a significant difference in perfusion between the PWS lesion and the contralateral healthy control skin (1132 ± 724 and 619 ± 478 PU, respectively, P < 0.01). Five minutes after V-PDT, the mean perfusion value of the 24 PWS lesions was 1246 ± 754 PU. There was no significant difference compared to the perfusion before V-PDT (P > 0.05). During V-PDT, the perfusion of seven PWS patients increased rapidly after initiation of V-PDT, reached a maximum within 10 min, lasted for several minutes, and slowly returned to a relatively lower level at the end of V-PDT. On the basis of these results, LSPI is capable of imaging PWS microvasculature and monitoring microvascular reactivity to V-PDT.

• Authors: Haixia Qiu, Yang Zhou, Ying Gu, Qing Ang, ShiYong Zhao, Ying Wang, Jing Zeng, Naiyan Huang
• Journal: Photochemistry and photobiology (Photochem Photobiol) 2012 Jul-Aug Vol. 88 Issue 4 Pg. 978-84 ISSN: 1751-1097 [Electronic] United States
• PMID: 22537271 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 Wiley Periodicals, Inc. Photochemistry and Photobiology © 2012 The American Society of Photobiology.

Chemical References

• Hematoporphyrins
• Photosensitizing Agents
• photocarcinorin

Topics

• Adolescent
• Adult
• Child
• Child, Preschool
• Diagnostic Imaging (methods)
• Female
• Hematoporphyrins (administration & dosage)
• Humans
• Lasers
• Light
• Male
• Microcirculation (radiation effects)
• Microvessels (pathology, radiation effects)
• Middle Aged
• Photochemotherapy
• Photosensitizing Agents (administration & dosage)
• Port-Wine Stain (drug therapy, pathology)
• Skin (blood supply, pathology, radiation effects)