In vitro and in vivo identification of a novel cytotoxic T lymphocyte epitope from Rv3425 of Mycobacterium tuberculosis.

Abstract

The identification of novel cytotoxic T lymphocyte (CTL) epitopes is important to analysis of the involvement of CD8(+) T cells in Mycobacterium tuberculosis infection as well as to the development of peptide vaccines. In this study, a novel CTL epitope from region of difference 11 encoded antigen Rv3425 was identified. Epitopes were predicted by the reversal immunology approach. Rv3425-p118 (LIASNVAGV) was identified as having relatively strong binding affinity and stability towards the HLA-A*0201 molecule. Peripheral blood mononuclear cells pulsed by this peptide were able to release interferon-γ in healthy donors (HLA-A*02(+) purified protein derivative(+)). In cytotoxicity assays in vitro and in vivo, Rv3425-p118 induced CTLs to specifically lyse the target cells. Therefore, this epitope could provide a subunit component for designing vaccines against Mycobacterium tuberculosis.

• Authors: Fei Chen, Ming-xia Zhai, Yu-huang Zhu, Yuan-ming Qi, Wen-jie Zhai, Yan-feng Gao
• Journal: Microbiology and immunology (Microbiol Immunol) Vol. 56 Issue 8 Pg. 548-53 (Aug 2012) ISSN: 1348-0421 [Electronic] Australia
• PMID: 22537173 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 The Societies and Blackwell Publishing Asia Pty Ltd.

Chemical References

• Bacterial Proteins
• Epitopes, T-Lymphocyte
• HLA-A*02:01 antigen
• HLA-A2 Antigen
• Interferon-gamma

Topics

• Animals
• Bacterial Proteins (immunology)
• Computational Biology
• Cytotoxicity Tests, Immunologic
• Epitope Mapping
• Epitopes, T-Lymphocyte (immunology)
• HLA-A2 Antigen (metabolism)
• Humans
• Interferon-gamma (metabolism)
• Leukocytes, Mononuclear (immunology)
• Mice
• Mice, Transgenic
• Mycobacterium tuberculosis (immunology)
• Protein Binding
• T-Lymphocytes, Cytotoxic (immunology)