Abstract |
From February 2010 to July 2011, 183 of 416 presumptive Klebsiella pneumoniae isolates with reduced susceptibility to third-generation cephalosporins from patients with lower respiratory tract infection were collected from seven tertiary hospitals in China. Phenotypic and genotypic methods were employed to characterize 158 extended-spectrum β-lactamase (ESBL)-producers. Among the 158 isolates analyzed, 134 (84.8%) harbored bla(CTX-M) , within which the most predominant ESBL gene was CTX-M-14 (49.4%), followed by CTX-M-15 (12.0%) and CTX-M-27 (10.8%). Also, 120 (75.9%) harbored bla(SHV) . One novel SHV variant, bla(SHV -142) with T18A and L35Q substitutions, was identified. Ninety-one isolates carried bla(TEM-1). An isolate containing bla(TEM-135) was first identified in Klebsiella spp. bla(KPC)-2) was detected in 5 isolates. More than one ESBL combination was detected in 18 isolates (11.4%). Fifty-four (34.2%) isolates demonstrated the multidrug resistant (MDR) phenotype. Seventy-four sequence types (STs) were identified, which showed large genetic background diversity in ESBL-producing K. pneumoniae isolates from the six areas. This is the first report on the high prevalence of CTX-M-27 in China with the possible transmission of a single clone (ST48). The correlated surveillance of organisms with MDR phenotype should be investigated in future.
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Authors | Shuchang An, Jichao Chen, Zhanwei Wang, Xiaorong Wang, Xixin Yan, Jihong Li, Yusheng Chen, Qi Wang, Xiaoling Xu, Jiabin Li, Jingping Yang, Hui Wang, Zhancheng Gao |
Journal | FEMS microbiology letters
(FEMS Microbiol Lett)
Vol. 332
Issue 2
Pg. 137-45
(Jul 2012)
ISSN: 1574-6968 [Electronic] England |
PMID | 22537112
(Publication Type: Letter, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Chemical References |
- Anti-Bacterial Agents
- DNA, Bacterial
- beta-Lactams
- beta-Lactamases
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- China
- DNA, Bacterial
(chemistry, genetics)
- Humans
- Klebsiella Infections
(microbiology)
- Klebsiella pneumoniae
(classification, enzymology, genetics, isolation & purification)
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Multilocus Sequence Typing
- Respiratory Tract Infections
(microbiology)
- Sequence Analysis, DNA
- beta-Lactamases
(metabolism)
- beta-Lactams
(pharmacology)
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