Acute and long-term effects of hyperthermia in B16-F10 melanoma cells.

Abstract

OBJECTIVE:

Hyperthermia uses exogenous heat induction as a cancer therapy. This work addresses the acute and long-term effects of hyperthermia in the highly metastatic melanoma cell line B16-F10.

MATERIALS AND METHODS:

Melanoma cells were submitted to one heat treatment, 45°C for 30 min, and thereafter were kept at 37°C for an additional period of 14 days. Cultures maintained at 37°C were used as control. Cultures were assessed for the heat shock reaction.

RESULTS:

Immediately after the heat shock, cells began a process of fast degradation, and, in the first 24 h, cultures showed decreased viability, alterations in cell morphology and F-actin cytoskeleton organization, significant reduction in the number of adherent cells, most of them in a process of late apoptosis, and an altered gene expression profile. A follow-up of two weeks after heat exposure showed that viability and number of adherent cells remained very low, with a high percentage of early apoptotic cells. Still, heat-treated cultures maintained a low but relatively constant population of cells in S and G(2)/M phases for a long period after heat exposure, evidencing the presence of metabolically active cells.

CONCLUSION:

The melanoma cell line B16-F10 is susceptible to one hyperthermia treatment at 45°C, with significant induced acute and long-term effects. However, a low but apparently stable percentage of metabolically active cells survived long after heat exposure.

• Authors: Mónica Pereira Garcia, José Roberto Tinoco Cavalheiro, Maria Helena Fernandes
• Journal: PloS one (PLoS One) Vol. 7 Issue 4 Pg. e35489 ( 2012) ISSN: 1932-6203 [Electronic] United States
• PMID: 22532856 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Apoptosis (physiology)
• Cell Cycle (physiology)
• Cell Line, Tumor
• Cell Proliferation
• Cell Shape (physiology)
• Cell Survival (physiology)
• Gene Expression
• Hot Temperature
• Hyperthermia, Induced
• Melanoma, Experimental (therapy)
• Mice
• Time
• Tumor Cells, Cultured