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Co-culture of mesenchymal stem cells with umbilical vein endothelial cells under hypoxic condition.

Abstract
By co-culturing humm mesenchymal stem cells (hMSCs) and human umbilical rein endothelial cells (HUVECs) under hypoxia and creating a microenvironment similar to that of transplanted hMSCs for the treatment of avascular ni ANFH, the effect of hMSCs on survival, apoptosis, migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) under the hypoxic condition were investigated in vitro. hMSCs and HUVECs were cultured and identified in vitro. Three kinds of conditioned media, CdM-CdM(NOR), CdM-CdM(HYP) and HUVEC-CdM(HYP) were prepared. HUVECs were cultured with these conditioned media under hypoxia. The survival rate, apoptosis rate, migration and angiogenesis of HUVECs were respectively detected by CCK-8, flow cytometry, Transwell and tube formation assay. The content of SDF-1α, VEGF and IL-6 in CdM was determined by ELISA. Our results showed that hMSCs and HUVECs were cultured and identified successfully. Compared with MSC-CdM(NOR) and HUVEC-CdM(HYP) groups, the survival rate, migration and angiogenesis of HUVECs in MSC-CdM(HYP) group were significantly increased while the apoptosis rate was declined (P<0.05). Moreover, the expression of SDF-1α, VEGF and IL-6 in MSC-CdM(HYP) group was up-regulated. Under hypoxia, the apoptosis of HUVECs was inhibited while survival, migration and angiogenesis were improved by co-culture of hMSCs and HUVECs. The underlying mechanism may be that hMSCs could secrete a number of cytokines and improve niche, which might be helpful in the treatment of femoral head necrosis.
AuthorsBo Zhang, Shuhua Yang, Yukun Zhang, Zhibo Sun, Weihua Xu, Shunan Ye
JournalJournal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 32 Issue 2 Pg. 173-180 (Apr 2012) ISSN: 1672-0733 [Print] China
PMID22528216 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cytokines
Topics
  • Adult
  • Cell Communication (physiology)
  • Cell Hypoxia (physiology)
  • Cell Movement (physiology)
  • Cell Survival (physiology)
  • Cells, Cultured
  • Coculture Techniques
  • Cytokines (metabolism)
  • Endothelial Cells (cytology, physiology)
  • Female
  • Humans
  • Male
  • Mesenchymal Stem Cells (cytology, physiology)
  • Neovascularization, Physiologic (physiology)
  • Umbilical Veins (cytology, physiology)

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