Abstract |
The use of virosomes as a vaccine platform has proven successful against several viruses. Here we examined the protective efficacy of a virosome-based vaccine consisting of avian influenza virus (AIV) A/Duck/Czech/56/H4N6 in chickens against a homologous AIV challenge. Virosomes adjuvanted with CpG-ODN or recombinant chicken interferon (IFN)-γ significantly reduced virus shedding after virus challenge. Furthermore, immunization with virosomes adjuvanted with CpG-ODN increased hemagglutination inhibition (HI) and virus-specific neutralizing serum antibodies, as well as virus-specific serum IgG and mucosal IgA responses. We also found a significant increase in the expression of type I and II interferon genes in the protected birds following virus challenge. In summary, this study demonstrated the ability of virosomes adjuvanted with CpG-ODN to reduce AIV shedding, and elicit virus-specific protective antibody responses in vaccinated birds.
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Authors | Amirul I Mallick, Raveendra R Kulkarni, Michael St Paul, Payvand Parvizi, Eva Nagy, Shahriar Behboudi, Shayan Sharif |
Journal | Viral immunology
(Viral Immunol)
Vol. 25
Issue 3
Pg. 226-31
(Jun 2012)
ISSN: 1557-8976 [Electronic] United States |
PMID | 22512311
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Neutralizing
- Antibodies, Viral
- CPG-oligonucleotide
- Influenza Vaccines
- Oligodeoxyribonucleotides
- Vaccines, Virosome
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Animals
- Antibodies, Neutralizing
(blood)
- Antibodies, Viral
(blood)
- Chickens
(immunology)
- Influenza A virus
(immunology)
- Influenza Vaccines
(administration & dosage)
- Influenza in Birds
(immunology, prevention & control, virology)
- Oligodeoxyribonucleotides
(administration & dosage, immunology)
- Poultry Diseases
(immunology, prevention & control)
- Vaccination
- Vaccines, Virosome
(administration & dosage, immunology)
- Virus Shedding
(immunology)
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