[The inhibitory effect of luteolin on inflammation in LPS-induced microglia].

Abstract

OBJECTIVE:

To study the inhibitory effect of Luteolin on LPS-induced BV-2 cell.

METHODS:

BV-2 cells were treated with LPS (0.1 microg/mL) for inflammation model; MTT assay was used to detect the viability of BV-2 cells; Nitric oxide (NO) was detected by the method of nitric acid reductase assay; Induce type II nitric oxide synthase (iNOS) enzyme activity was determined by type of nitric oxide synthase assay;TLR4 protein expression was examined by the Western Blot analysis.

RESULTS:

Luteolin significantly decreased the NO production and TLR4 protein expression as well as iNOS activity in LPS-activated microglial cell.

CONCLUSION:

LPS induced activation of microglia lead to inflammatory response and its mechanism may be related to inhibiting TLR4 signaling pathway.

• Authors: Feng Huang, Miao-Miao Zhu, Hua-Ming Deng, Zai-Jun Zhang, Li Yang, Fei Xiao, Ying Xiao, Hong Nie
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 34 Issue 11 Pg. 1746-9 (Nov 2011) ISSN: 1001-4454 [Print] China
• PMID: 22506402 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Lipopolysaccharides
• Toll-Like Receptor 4
• Nitric Oxide
• Nitric Oxide Synthase
• Luteolin

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Blotting, Western
• Cell Line
• Cell Survival (drug effects)
• Enzyme Activation (drug effects)
• Gene Expression Regulation, Enzymologic (drug effects)
• Inflammation (chemically induced, metabolism, prevention & control)
• Lipopolysaccharides (administration & dosage, pharmacology)
• Luteolin (pharmacology)
• Mice
• Microglia (drug effects, metabolism, pathology)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase (metabolism)
• Signal Transduction (drug effects)
• Toll-Like Receptor 4 (metabolism)