[Mechanism of Dahuang Zhechong pill against atherosclerosis induced by balloon angioplasty in rabbits].

Abstract

OBJECTIVE:

To study the mechanism of Dahuang Zhechong pill (DHZCP) against atherosclerosis induced by balloon angioplasty in rabbits.

METHODS:

Atherosclerosis model was established by the combination of balloon angioplasty-induced endothelial injury and high cholesterol feeding in rabbit. Male New Zealand rabbits were divided into six groups randomly: normal control, sham, model, positive control and two doses of DHZCP-treated groups. Rabbits in DHZCP-treated groups were intragastrically administered 0.9 and 1.8 g/kg DHZCP for 60 days respectively,and rabbits in positive control group were given 0.5 g/kg Danshen. MDA, NO levels and SOD activity in serum, and MPO activity in the vascular wall were determined with spectrophotometry. Expressions of proliferating cell nuclear antigen (PCNA) and BCL-2 in the vascular wall were detected by SP immuohistochemical technique.

RESULTS:

Compared with the model group, DHZCP significantly reduced serum MDA level and MPO activity in the vascular wall, increased serum NO level and SOD activity,and inhibited PCNA and BCL-2 expressions in the vascular wall.

CONCLUSION:

DHZCP inhibits the formation and development of atherosclerosis through anti-oxidative action, protecting endothelium from injury,inhibiting proliferation and promoting apoptosis of vascular smooth muscle cells.

• Authors: Chun-Jie Han, Jun-Tian Liu, Ying Zhang, Ming Li, Xiao-Ming Pang, Jun-Jun Mao
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 34 Issue 12 Pg. 1919-22 (Dec 2011) ISSN: 1001-4454 [Print] China
• PMID: 22500432 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dahuang Zhechong pill
• Drug Combinations
• Drugs, Chinese Herbal
• Proliferating Cell Nuclear Antigen
• Malondialdehyde
• Superoxide Dismutase

Topics

• Animals
• Aorta, Thoracic (metabolism, pathology)
• Apoptosis (drug effects)
• Atherosclerosis (blood, metabolism, pathology, prevention & control)
• Cell Proliferation (drug effects)
• Cockroaches (chemistry)
• Disease Models, Animal
• Drug Combinations
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Immunohistochemistry
• Male
• Malondialdehyde (blood)
• Muscle, Smooth, Vascular (cytology, drug effects, metabolism)
• Myocytes, Smooth Muscle (drug effects, metabolism, pathology)
• Proliferating Cell Nuclear Antigen (metabolism)
• Rabbits
• Random Allocation
• Rheum (chemistry)
• Superoxide Dismutase (blood)