Glucose-induced dysmorphogenesis in the cultured rat conceptus: prevention by supplementation with myo-inositol.

Abstract

Growth retardation and dysmorphogenesis in the rat conceptus are accompanied by diminished tissue myo-inositol following culture from day 9.5 to 11.5 of development in the presence of increased amounts of glucose. Reductions of myo-inositol and increased malformations are not corrected by aldose reductase inhibitors. In contrast, supplementation of culture medium with myo-inositol (1.5 mg/ml) restores tissue myo-inositol content, lowers the incidence of dysmorphogenic embryos (from 51.1 to 28.6%, P less than 0.001) and reduces the incidence of neural tube defects to control levels (from 33.3 to 6%, P less than 0.001). These results suggest that myo-inositol depletion is involved in the mechanism of diabetic embryopathy.

• Authors: M Hod, S Star, J Passonneau, T G Unterman, N Freinkel
• Journal: Israel journal of medical sciences (Isr J Med Sci) Vol. 26 Issue 10 Pg. 541-4 (Oct 1990) ISSN: 0021-2180 [Print] Israel
• PMID: 2249926 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Culture Media
• Inositol
• Sorbitol
• Glucose

Topics

• Animals
• Congenital Abnormalities (etiology, metabolism, prevention & control)
• Culture Media
• Culture Techniques
• Disease Models, Animal
• Embryo, Mammalian (chemistry, drug effects, metabolism)
• Female
• Glucose (metabolism, pharmacology)
• Humans
• Hyperglycemia (complications, metabolism)
• Inositol (metabolism, pharmacology)
• Pregnancy
• Pregnancy in Diabetics (metabolism)
• Rats
• Rats, Inbred Strains
• Sorbitol (metabolism)