Abstract | AIM: METHODS: Immunohistochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of MPG gene and protein in 128 glioma and 10 non-neoplastic brain tissues. RESULTS: MPG gene expression level in glioma tissues was significantly higher than that in non-neoplastic brain tissues (P < 0.001). Immunohistochemistry also showed that MPG protein was over-expressed in glioma tissues, which was consistent with the resutls of Western blot analysis. Additionally, the expression levels of MPG gene and protein both increase from grade I to grade IV glioma according to the results of real-time PCR, immunohistochemistry and western blot analysis. Moreover, the survival rate of MPG-positive patients was significantly lower than that of MPG-negative patients (P < 0.001). We further confirmed that the over-expression of MPG was a significant and independent prognostic indicator in glioma by multivariate analysis (P < 0.001). CONCLUSIONS: Our data showed the over-expression of MPG gene and protein in human gliomas, and also suggested for the first time that MPG be an unfavorable independent prognostic indicator for glioma patients.
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Authors | Ce Liu, Yanyang Tu, Jun Yuan, Xinggang Mao, Shiming He, Liang Wang, Guoqiang Fu, Jianhai Zong, Yongsheng Zhang |
Journal | Journal of biomedicine & biotechnology
(J Biomed Biotechnol)
Vol. 2012
Pg. 760679
( 2012)
ISSN: 1110-7251 [Electronic] United States |
PMID | 22496614
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Glycosylases
- DNA-3-methyladenine glycosidase II
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Topics |
- Adolescent
- Adult
- Aged
- Blotting, Western
- Brain Neoplasms
(chemistry, enzymology)
- Case-Control Studies
- Child
- DNA Glycosylases
(biosynthesis, genetics)
- Female
- Glioma
(chemistry, enzymology)
- Humans
- Immunohistochemistry
- Kaplan-Meier Estimate
- Male
- Middle Aged
- Multivariate Analysis
- Prognosis
- Proportional Hazards Models
- Real-Time Polymerase Chain Reaction
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