HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Aberrant expression of N-methylpurine-DNA glycosylase influences patient survival in malignant gliomas.

AbstractAIM:
To examine the expression of N-methylpurine-DNA glycosylase (MPG) gene and protein in glioma samples with different WHO grades and its association with patients' survival.
METHODS:
Immunohistochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of MPG gene and protein in 128 glioma and 10 non-neoplastic brain tissues.
RESULTS:
MPG gene expression level in glioma tissues was significantly higher than that in non-neoplastic brain tissues (P < 0.001). Immunohistochemistry also showed that MPG protein was over-expressed in glioma tissues, which was consistent with the resutls of Western blot analysis. Additionally, the expression levels of MPG gene and protein both increase from grade I to grade IV glioma according to the results of real-time PCR, immunohistochemistry and western blot analysis. Moreover, the survival rate of MPG-positive patients was significantly lower than that of MPG-negative patients (P < 0.001). We further confirmed that the over-expression of MPG was a significant and independent prognostic indicator in glioma by multivariate analysis (P < 0.001).
CONCLUSIONS:
Our data showed the over-expression of MPG gene and protein in human gliomas, and also suggested for the first time that MPG be an unfavorable independent prognostic indicator for glioma patients.
AuthorsCe Liu, Yanyang Tu, Jun Yuan, Xinggang Mao, Shiming He, Liang Wang, Guoqiang Fu, Jianhai Zong, Yongsheng Zhang
JournalJournal of biomedicine & biotechnology (J Biomed Biotechnol) Vol. 2012 Pg. 760679 ( 2012) ISSN: 1110-7251 [Electronic] United States
PMID22496614 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA Glycosylases
  • DNA-3-methyladenine glycosidase II
Topics
  • Adolescent
  • Adult
  • Aged
  • Blotting, Western
  • Brain Neoplasms (chemistry, enzymology)
  • Case-Control Studies
  • Child
  • DNA Glycosylases (biosynthesis, genetics)
  • Female
  • Glioma (chemistry, enzymology)
  • Humans
  • Immunohistochemistry
  • Kaplan-Meier Estimate
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Prognosis
  • Proportional Hazards Models
  • Real-Time Polymerase Chain Reaction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: