Salidroside protects human erythrocytes against hydrogen peroxide-induced apoptosis.

Abstract

Rhodiola rosea is a commonly used folk medicine for the treatment of high altitude sickness, mountain malhypoxia, and anoxia. Its active ingredient, salidroside [2-(4-hydroxyphenyl)ethyl β-D-glucopyranoside (1)], has been reported to have a broad spectrum of biological effects. However, the protective role of 1 in human erythrocytes remains unclear. This study therefore has investigated the effects of 1 on oxidative stress-induced apoptosis in human erythrocytes (also known as eryptosis or erythroptosis). Compound 1 increased cell survival significantly and prevented human erythrocytes from undergoing eryptosis/erythroptosis mediated by H(2)O(2), as confirmed by the decreased expression of phosphatidylserine on the cell surface and reduced leakage of calcein through the damaged membrane. Mechanistically, 1 was found to exert its protective effects through its antioxidative activity and the inhibition of caspase-3 activation and stress-induced intracellular Ca(2+) rise in a dose-dependent manner. Compound 1 is a protective agent in human erythrocytes against oxidative stress and may be a good adaptogen to enhance the body's resistance to stress and fatigue.

• Authors: Erin Wei Qian, Daniel Tianfang Ge, Siu-Kai Kong
• Journal: Journal of natural products (J Nat Prod) Vol. 75 Issue 4 Pg. 531-7 (Apr 27 2012) ISSN: 1520-6025 [Electronic] United States
• PMID: 22483064 (Publication Type: Journal Article)
• Copyright: © 2012 American Chemical Society and American Society of Pharmacognosy

Chemical References

• Antioxidants
• Glucosides
• Phenols
• Hydrogen Peroxide
• rhodioloside
• Calcium

Topics

• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Calcium (analysis)
• Dose-Response Relationship, Drug
• Erythrocytes (metabolism)
• Glucosides (blood, pharmacology)
• Humans
• Hydrogen Peroxide (pharmacology)
• Molecular Structure
• Oxidative Stress (drug effects)
• Phenols (blood, pharmacology)
• Rhodiola (chemistry)