Investigation of genome instability in exfoliated colonic epithelial cells and in mitogen-stimulated lymphocytes of patients with ulcerative colitis.

Abstract

OBJECTIVE:

The present study aimed to evaluate the micronucleus (MN), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) in the mitogen-stimulated lymphocytes of patients with ulcerative colitis (UC). In addition, we assessed MN frequency in exfoliated colonic epithelial cells obtained from both the diseased and healthy colonic mucosa of patients.

DESIGN:

The study was conducted in 22 newly diagnosed patients with UC and in 22 healthy controls. MN, NPB and NBUD values scored in binucleated (BN) cells were obtained from the mitogen-stimulated lymphocytes of patients and control subjects. In addition, the MN values in exfoliated epithelial cells obtained from the diseased and healthy colonic mucosa of patients were evaluated.

RESULTS:

We found significantly higher MN, NPB and NBUD frequencies in the BN cells of patients with UC than in those of the control subjects (1.61 ± 0.75 vs. 0.89 ± 0.29, 3.93 ± 1.91 vs. 1.39 ± 1.10, and 1.55 ± 0.89 vs. 0.64 ± 0.48, p = 0.001). Also, a statistically significant difference was found between MN frequencies obtained from the diseased and healthy colonic mucosa of patients (1.07 ± 0.46 vs. 0.59 ± 0.21, p = 0.001). No significant relationship was found between age and MN frequency in patients with UC (r = 0.076, p = 0.735).

CONCLUSION:

Increased MN, NPB and NBUD frequencies observed in both the lymphocytes and exfoliated colonic epithelial cells obtained from patients with UC may reflect genomic instability.

• Authors: Ahmet Karaman, Zuhal Hamurcu, Hamiyet Donmez, Nazmiye Bitken, Hatice Karaman, Mevlut Baskol, Sebnem Gursoy, Kadri Guven, Omer Ozbakir, Mehmet Yucesoy
• Journal: Digestion (Digestion) Vol. 85 Issue 3 Pg. 228-35 ( 2012) ISSN: 1421-9867 [Electronic] Switzerland
• PMID: 22472630 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 S. Karger AG, Basel.

Topics

• Adult
• Aged
• Case-Control Studies
• Colitis, Ulcerative (pathology)
• Colon (pathology)
• Epithelial Cells (ultrastructure)
• Female
• Genomic Instability
• Humans
• Intestinal Mucosa (pathology)
• Lymphocyte Activation
• Lymphocytes (ultrastructure)
• Male
• Micronucleus Tests
• Middle Aged
• Young Adult