Suppression of astrocyte lineage in adult hippocampal progenitor cells expressing hippocampal cholinergic neurostimulating Peptide precursor in an in vivo ischemic model.

Abstract

Hippocampal cholinergic neurostimulating peptide (HCNP) is known to promote differentiation of septohippocampal cholinergic neurons. The HCNP precursor protein (HCNP-pp) may play several roles, for example, as an ATP-binding protein, a Raf kinase inhibitor protein, and a phosphatidylethanolamine-binding protein, as well as a precursor for HCNP. This study therefore aimed to elucidate the involvement of HCNP-pp in specific neural lineages after stroke using a hypoxic-ischemic (HI) rat model of brain ischemia. The specific neural lineages in the hippocampus were investigated 14 days after ischemia. Some bromodeoxyuridine (BrdU)(+) neural progenitor cells in the hippocampus of hypoxic, HI, or sham-operated rats expressed HCNP-pp. Almost half of the BrdU(+)/HCNP-pp(+) cells also expressed the oligodendrocyte lineage marker 2',3'-cyclic nucleotide 3'-phosphodiesterase, whereas only a few BrdU(+)/HCNP-pp(+) cells in the hippocampus in HI brains expressed the neuronal lineage marker, doublecortin (DCX). Interestingly, no BrdU(+)/HCNP-pp(+) progenitor cells in hypoxic, HI, or sham-operated brains expressed the astrocyte lineage marker, glial fibrillary acidic protein. Together with previous in vitro data, the results of this study suggest that the expression level of HCNP-pp regulates the differentiation of neural progenitor cells into specific neural lineages in the HI hippocampus, indicating that neural stem cell fate can be controlled via the HCNP-pp mediating pathway.

• Authors: Takanari Toyoda, Noriyuki Matsukawa, Takafumi Sagisaka, Norihiko Uematsu, Tetsuko Kanamori, Daisuke Kato, Masayuki Mizuno, Hiroaki Wake, Hideki Hida, Cesario V Borlongan, Kosei Ojika
• Journal: Cell transplantation (Cell Transplant) Vol. 21 Issue 10 Pg. 2159-69 ( 2012) ISSN: 1555-3892 [Electronic] United States
• PMID: 22469272 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dcx protein, rat
• Doublecortin Protein
• Phosphatidylethanolamine Binding Protein

Topics

• Animals
• Astrocytes (cytology, metabolism)
• Brain Ischemia (metabolism, pathology)
• Cell Differentiation (physiology)
• Cell Growth Processes (physiology)
• Cell Lineage
• Disease Models, Animal
• Doublecortin Protein
• Female
• Immunohistochemistry
• Male
• Neural Stem Cells (cytology, metabolism)
• Phosphatidylethanolamine Binding Protein (biosynthesis)
• Rats
• Rats, Sprague-Dawley