Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers.

Abstract

AIM:

To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.

METHODS:

We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma. A set of proteins was validated with immunoblotting.

RESULTS:

We identified 30 different proteins involved in various biological processes: metabolism, development, death, response to stress, cell cycle, cell communication, transport, and cell motility. Eight proteins were chosen for further validation by immunoblotting. Our results show that gastrokine-1, 39S ribosomal protein L12 (mitochondrial precursor), plasma cell-induced resident endoplasmic reticulum protein, and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples. On the other hand, septin-2, ubiquitin-conjugating enzyme E2 N, and transaldolase were significantly overexpressed. Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.

CONCLUSION:

This work presents a set of possible diagnostic biomarkers, validated for the first time. It might contribute to the efforts of understanding gastric cancer carcinogenesis.

• Authors: Nina Kočevar, Federico Odreman, Alessandro Vindigni, Snježana Frković Grazio, Radovan Komel
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 18 Issue 11 Pg. 1216-28 (Mar 21 2012) ISSN: 2219-2840 [Electronic] United States
• PMID: 22468085 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (metabolism)
• Female
• Humans
• Immunoblotting (methods)
• Male
• Mass Spectrometry (methods)
• Middle Aged
• Proteomics (methods)
• Reproducibility of Results
• Stomach Neoplasms (diagnosis, genetics, metabolism, pathology)