Xanthorrhizol induced DNA fragmentation in HepG2 cells involving Bcl-2 family proteins.

Abstract

Xanthorrhizol is a plant-derived pharmacologically active sesquiterpenoid compound isolated from Curcuma xanthorrhiza. Previously, we have reported that xanthorrhizol inhibited the proliferation of HepG2 human hepatoma cells by inducing apoptotic cell death via caspase activation. Here, we attempt to further elucidate the mode of action of xanthorrhizol. Apoptosis in xanthorrhizol-treated HepG2 cells as observed by scanning electron microscopy was accompanied by truncation of BID; reduction of both anti-apoptotic Bcl-2 and Bcl-X(L) expression; cleavage of PARP and DFF45/ICAD proteins and DNA fragmentation. Taken together, these results suggest xanthorrhizol as a potent antiproliferative agent on HepG2 cells by inducing apoptosis via Bcl-2 family members. Hence we proposed that xanthorrhizol could be used as an anti-liver cancer drug for future studies.

• Authors: Thiam-Tsui Tee, Yew-Hoong Cheah, Nallappan Meenakshii, Mohd Yusof Mohd Sharom, Lope Pihie Azimahtol Hawariah
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 420 Issue 4 Pg. 834-8 (Apr 20 2012) ISSN: 1090-2104 [Electronic] United States
• PMID: 22465013 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Antineoplastic Agents
• Phenols
• Proto-Oncogene Proteins c-bcl-2
• xanthorrhizol

Topics

• Antineoplastic Agents (pharmacology)
• DNA Fragmentation
• Hep G2 Cells
• Humans
• Liver Neoplasms (metabolism)
• Phenols (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)