Old and new diagnostic approaches for Q fever diagnosis: correlation among serological (CFT, ELISA) and molecular analyses.

Abstract

The objective of this study was to evaluate the performance of the complement fixation test (CFT) with respect to ELISA for the serological diagnosis of Q fever and to assess the role of serology as a tool for the identification of the shedder status. During 2009-2010, sera from 9635 bovines and 3872 small ruminants (3057 goats and 815 sheep) were collected and analyzed with CFT and ELISA. In addition, 2256 bovine, 139 caprine and 72 ovine samples (individual and bulk tank milk samples, fetuses, vaginal swabs and placentae) were analyzed with a real-time PCR kit. The relative sensitivity (Se) and specificity (Sp) of CFT with respect to ELISA were Se 26.56% and Sp 99.71% for cattle and Se 9.96% and Sp 99.94% for small ruminants. To evaluate the correlation between serum-positive status and shedder status, the ELISA, CFT and real-time PCR results were compared. Due to the sampling method and the data storage system, the analysis of individual associations between the serological and molecular tests was possible only for some of the bovine samples. From a statistical point of view, no agreement was observed between the serological and molecular results obtained for fetus and vaginal swab samples. Slightly better agreement was observed between the serological and molecular results obtained for the individual milk samples and between the serological (at least one positive in the examined group) and molecular results for the bulk tank milk (BTM) samples. The CFT results exhibited a better correlation with the shedder status than did the ELISA results.

• Authors: A Natale, G Bucci, K Capello, A Barberio, A Tavella, S Nardelli, S Marangon, L Ceglie
• Journal: Comparative immunology, microbiology and infectious diseases (Comp Immunol Microbiol Infect Dis) Vol. 35 Issue 4 Pg. 375-9 (Jul 2012) ISSN: 1878-1667 [Electronic] England
• PMID: 22463984 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Topics

• Animals
• Cattle
• Cattle Diseases (diagnosis, microbiology)
• Complement Fixation Tests
• Coxiella burnetii (genetics, isolation & purification)
• Enzyme-Linked Immunosorbent Assay
• Female
• Fetus (microbiology)
• Goat Diseases (diagnosis, microbiology)
• Goats
• Milk (microbiology)
• Placenta (microbiology)
• Pregnancy
• Q Fever (diagnosis, microbiology, veterinary)
• Real-Time Polymerase Chain Reaction
• Sensitivity and Specificity
• Sheep
• Sheep Diseases (diagnosis, microbiology)