Abstract |
Ca(2+)-mediated signal transduction of group I metabotropic glutamate receptors (ImGluR) was studied in the brain of young (15 days) and old rats (90 days) exposed to severe hypobaric hypoxia on gestation days 14-16. Changes in the concentration of bound intracellular Ca(2+) (Ca(2+) response) were evaluated after repeated application of a selective ImGluR agonist 3,5-dihydroxyphenylglycine ( DHPG) to cultured brain slices. Primary application of DHPG for 2 min induced a negative Ca(2+) response in slices from 15-day-old intact animals, while repeated application caused a positive response. In slices from 90-day-old control animals, both responses were negative. In slices from rats of both age groups subjected to severe prenatal hypobaric hypoxia, both responses were mainly positive, but short-term negative components were present in adult animals. Our results suggest that severe hypobaric hypoxia changes the balance between the two constitutive signal pathways triggered by ImGluR ( inosine triphosphate and diacylglycerol pathways). This procedure is followed by the increased influx of extracellular Ca(2+) (as compared to Ca(2+) release from the intracellular stores). This imbalance is particularly pronounced at the early stage of ontogeny.
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Authors | E I Tyul'kova, D G Semenov, L A Vataeva, A V Belyakov, M O Samoilov |
Journal | Bulletin of experimental biology and medicine
(Bull Exp Biol Med)
Vol. 151
Issue 3
Pg. 275-7
(Jul 2011)
ISSN: 1573-8221 [Electronic] United States |
PMID | 22451864
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Diglycerides
- Excitatory Amino Acid Agents
- Receptors, Metabotropic Glutamate
- Resorcinols
- Inosine Triphosphate
- 3,5-dihydroxyphenylglycine
- Calcium
- Glycine
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Topics |
- Animals
- Brain
(metabolism)
- Calcium
(metabolism)
- Diglycerides
(metabolism)
- Excitatory Amino Acid Agents
(pharmacology)
- Female
- Fetal Hypoxia
(metabolism)
- Glycine
(analogs & derivatives, pharmacology)
- Inosine Triphosphate
(metabolism)
- Male
- Pregnancy
- Prenatal Exposure Delayed Effects
- Rats
- Rats, Wistar
- Receptors, Metabotropic Glutamate
(agonists, metabolism)
- Resorcinols
(pharmacology)
- Signal Transduction
- Tissue Culture Techniques
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