Infliximab enhances the therapeutic effectiveness of octreotide on acute necrotizing pancreatitis in rat model.

Abstract

OBJECTIVES:

To investigate the synergistic activity of infliximab to the therapeutic effectiveness of octreotide in a rat model of acute necrotizing pancreatitis (ANP).

METHODS:

Forty Sprague-Dawley rats were randomly divided into sham-operated group (SO), ANP group (ANP), octreotide group (OG), infliximab group (IG), and combination group (CG) (n = 8 in each group). The ANP model was induced by biliopancreatic duct injection with 4.5% of sodium taurocholate solution. Rats of the OG, IG, and CG were given a tail vein injection of octreotide (10 μg/kg), infliximab (8 mg/kg), and infliximab (8 mg/kg), respectively, combined with octreotide (10 μg/kg) at 6 hours after modeling. All rats in each group were killed at 24 hours after modeling. Serum biochemical indicator and partial pressure of arterial oxygen/fraction of inspired oxygen (PaO2/FiO2) of rats were determined. Pathological severity score of organs were evaluated.

RESULTS:

The serum biochemical indicator and organs' pathology score of OG , IG, and CG were obviously lower than those in the ANP group, and those in the CG were the lowest (P < 0.05). The PaO2/FiO2 levels in the OG, IG, and CG were significantly higher than that in the ANP group (P < 0.05).

CONCLUSION:

Infliximab could significantly lower the serum biochemical indicator, improve organs' function, and enhance the therapeutic effectiveness of octreotide on ANP.

• Authors: Yao-Xing Huang, Wei-Dong Li, Lin Jia, Jia-Hua Qiu, Shu-Man Jiang, Ya Ou, Hong Du
• Journal: Pancreas (Pancreas) Vol. 41 Issue 6 Pg. 849-54 (Aug 2012) ISSN: 1536-4828 [Electronic] United States
• PMID: 22450369 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Biomarkers
• Protective Agents
• Taurocholic Acid
• Infliximab
• Octreotide

Topics

• Animals
• Antibodies, Monoclonal (administration & dosage, pharmacology)
• Biomarkers (blood)
• Disease Models, Animal
• Drug Synergism
• Drug Therapy, Combination
• Female
• Infliximab
• Injections, Intravenous
• Liver Failure (etiology, prevention & control)
• Multiple Organ Failure (etiology, pathology, prevention & control)
• Octreotide (administration & dosage, pharmacology)
• Pancreatitis, Acute Necrotizing (blood, chemically induced, complications, drug therapy, pathology)
• Protective Agents (administration & dosage, pharmacology)
• Rats
• Rats, Sprague-Dawley
• Renal Insufficiency (etiology, prevention & control)
• Respiratory Insufficiency (etiology, prevention & control)
• Severity of Illness Index
• Taurocholic Acid
• Time Factors