Diagnostic utility of CD117, CD133, SALL4, OCT4, TCL1 and glypican-3 in malignant germ cell tumors of the ovary.

Abstract

AIM:

Primary ovarian malignant germ cell tumors (OMGCTs) are rare and difficult to diagnose. Immunohistochemistry can help in the diagnosis and development of new management strategies. The aim of this study was to investigate the frequency of CD117, CD133, SALL4, OCT4, TCL1 and glypican-3 marker expression in OMGCTs.

MATERIAL AND METHODS:

We examined the expression of six markers in 87 (85 pure and two mixed OMGCTs) cases of OMGCT using immunohistochemical staining. Staining was graded in a semiquantitative manner as follows: negative (no staining), 1+ (1-30% staining), 2+ (31-60% staining), or 3+ (>60% staining).

RESULTS:

All 27 dysgerminomas and all 31 YSTs showed CD117 expression, with only nine (29%) positively stained in immature teratomas. SALL4 and glypican-3 were strongly positive in 100 and 79.3%, respectively, of YSTs. All dysgerminomas were positive for OCT4, whereas all YSTs and immature teratomas were negative. 100% of dysgerminomas were positive for TCL1, but all immature teratomas were negative. CD133 expression showed generally the same tendency in the 3 OMGCTs.

CONCLUSION:

CD117 can be used as a diagnostic marker for dysgerminoma and YST. SALL4 is a more sensitive and specific marker for YSTs than glypican-3. SALL4 and OCT4 are useful in distinguishing YST from dysgerminoma.

• Authors: Dat Tien Trinh, Kiyosumi Shibata, Tomoya Hirosawa, Tomokazu Umezu, Mika Mizuno, Hiroaki Kajiyama, Fumitaka Kikkawa
• Journal: The journal of obstetrics and gynaecology research (J Obstet Gynaecol Res) Vol. 38 Issue 5 Pg. 841-8 (May 2012) ISSN: 1447-0756 [Electronic] Australia
• PMID: 22448662 (Publication Type: Journal Article)
• Copyright: © 2012 The Authors. Journal of Obstetrics and Gynaecology Research © 2012 Japan Society of Obstetrics and Gynecology.

Chemical References

• AC133 Antigen
• Antigens, CD
• Biomarkers, Tumor
• GPC3 protein, human
• Glycoproteins
• Glypicans
• Octamer Transcription Factor-3
• POU5F1 protein, human
• PROM1 protein, human
• Peptides
• Proto-Oncogene Proteins
• SALL4 protein, human
• TCL1A protein, human
• Transcription Factors
• Proto-Oncogene Proteins c-kit

Topics

• AC133 Antigen
• Adolescent
• Adult
• Aged
• Antigens, CD (metabolism)
• Biomarkers, Tumor (metabolism)
• Diagnosis, Differential
• Female
• Glycoproteins (metabolism)
• Glypicans (metabolism)
• Humans
• Immunohistochemistry
• Middle Aged
• Neoplasms, Germ Cell and Embryonal (diagnosis, metabolism)
• Octamer Transcription Factor-3 (metabolism)
• Ovarian Neoplasms (diagnosis, metabolism)
• Peptides (metabolism)
• Proto-Oncogene Proteins (metabolism)
• Proto-Oncogene Proteins c-kit (metabolism)
• Sensitivity and Specificity
• Transcription Factors (metabolism)