The prognostic value of Ki-67 expression in penile squamous cell carcinoma.

Abstract

AIMS:

To determine whether Ki-67 immunoexpression in penile squamous cell carcinoma (PSCC) has a prognostic value and correlates with lymph node metastasis, human papillomavirus (HPV) infection and patient survival.

METHODS:

148 formalin-fixed paraffin-embedded PSCC samples were tissue-microarrayed, including 97 usual-type SCCs, 17 basaloid, 15 pure verrucous carcinomas, 2 warty and 17 mixed-type tumours. All samples were immunostained for Ki-67 protein. HPV DNA was detected with INNO-LiPA assay. Follow-up data were available for 134 patients.

RESULTS:

Ki-67 was strongly expressed in 57/148 (38.5%) of PSCCs. Different cancer subtypes showed significant difference in Ki-67 expression (p<0.0001) with highest positivity in basaloid, 16/17 (94%), followed by usual type, 38/97 (39%) and lack of Ki-67 positive cases within verrucous tumours, 0/15. Ki-67 positively correlated with high-risk HPV (p<0.0001) and showed good specificity (84%) but low sensitivity (61%) for high-risk HPV detection. Ki-67 protein strongly positively correlated with tumour grade (p<0.0001) but not with stage (p=0.2193), or lymph node status (p=0.7366). Ki-67 showed no prognostic value for cancer-specific survival (HR=1.00, 95%, CI 0.99 to 1.02, p=0.54) or overall survival (HR=1.00, 95%, CI 0.99 to 1.02, p=0.45). High tumour stage, lymph node metastasis, high tumour grade and age at diagnosis were all independent prognostic factors for cancer-specific survival and overall survival.

CONCLUSIONS:

Ki-67 is only a moderate surrogate marker for HPV infection in PSCC. It does not show prognostic value for cancer-specific survival and overall survival in PSCC.

• Authors: Elzbieta Stankiewicz, Mansum Ng, Jack Cuzick, David Mesher, Nick Watkin, Wayne Lam, Cathy Corbishley, Daniel M Berney
• Journal: Journal of clinical pathology (J Clin Pathol) Vol. 65 Issue 6 Pg. 534-7 (Jun 2012) ISSN: 1472-4146 [Electronic] England
• PMID: 22447920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• DNA, Viral
• Ki-67 Antigen

Topics

• Alphapapillomavirus (genetics, isolation & purification)
• Biomarkers, Tumor (metabolism)
• Carcinoma, Squamous Cell (diagnosis, metabolism, mortality, virology)
• DNA, Viral (analysis)
• Humans
• Ki-67 Antigen (metabolism)
• Male
• Neoplasm Grading
• Papillomavirus Infections (complications, diagnosis, metabolism, mortality)
• Penile Neoplasms (diagnosis, metabolism, mortality, virology)
• Prognosis
• Survival Rate
• Tissue Array Analysis
• United Kingdom (epidemiology)